Antibody Expression
Antibody Expression - Related Videos
04:56
Antibody-Mediated Neutralization of Coronavirus Spike Protein-Expressing Pseudoviruses Using Patient Serum
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2026
Source: Jamieson, T. R., et al. Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection. J. Vis. Exp. (2021)This video demonstrates the antibody-mediated neutralization of pseudoviruses carrying coronavirus spike proteins using patient serum, studied through high-throughput fluorescent imaging of infected epithelial cell monolayers.
11:57
Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
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Cited by 2 •
2016
Controlling protein expression is not only essential to every organism alive, but also an important strategy to investigate protein functions in cellular models. The protocol presented shows the application of antibody interference in mammalian cells including primary hippocampal neurons and demonstrates the use of three-dimensional reconstructions in studying protein function.
03:02
Antibody Microarray: A Technique to Study the Protein Expression of miRNA Treated Lung Cancer Cells
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2023
This video describes the technique to study the protein expression in miRNA transfected lung cancer cells by using antibody microarray to measure specific gene expression. In the featured protocol, we perform antibody microarray for the identification of the activity of a combinatorial miRNA treatment in halting cell cycle and angiogenesis.
12:55
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Cited by 33 •
2015
A method is described with visual accompaniment for conducting scalable, high throughput selections from phage-displayed combinatorial synthetic antibody libraries against hundreds of antigens simultaneously. Using this parallel approach, we have isolated antibody fragments that exhibit high affinity and specificity for diverse antigens that are functional in standard immunoassays.
13:21
Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
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2023
Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4 1 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Department of Urology, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Polyclonal antibodies are defined as a collection of antibodies directed against...
FAQs
Activated B cells differentiate into plasma cells, which intensify transcription of immunoglobulin genes. The resulting heavy- and light-chain proteins are produced, assembled into complete immunoglobulin molecules, and secreted. This sequence links cellular differentiation with both the amount of antibody available and the molecular form that can participate in immune recognition or pathogen control.
Antibody expression requires coordinated production of heavy- and light-chain proteins. These components must assemble into complete immunoglobulin molecules before secretion by plasma cells or engineered host cells. Studying their expression therefore helps researchers relate antibody abundance and molecular structure to functional questions, including antigen characterization, neutralization, and the effectiveness of immune responses.
In vivo expression follows immune-cell activation and plasma-cell differentiation. Biotechnology instead uses cloned antibody genes introduced into a host system, such as mammalian cells. The host then produces the encoded chains and supports their folding, assembly, and modification. Comparing these settings helps researchers obtain antibodies experimentally while examining how production context may relate to antibody structure.
A typical engineered workflow begins by introducing cloned antibody genes into a suitable host-cell system. The host cells then produce the encoded heavy and light chains, fold and assemble them, and modify the resulting molecules. The expressed antibody can subsequently be obtained for experimental studies, including antigen characterization, pathogen detection, neutralization analysis, or development-oriented investigations.
Expressed antibodies support several complementary activities: detecting pathogens, characterizing antigens, testing whether antibodies neutralize targets, developing diagnostic tools, and evaluating therapeutic possibilities. The same production capability can provide material for studying immune recognition and for comparing antibody behavior across experimental settings. Its value depends on obtaining molecules that are sufficiently representative for the intended investigation.
Expression analysis connects antibody structure and abundance with the outcome of an immune response. In infection research, this perspective can help investigators examine relationships between antibody production and protective activity. It can also reveal patterns associated with dysregulated responses. Consequently, antibody expression provides a molecular and cellular context for interpreting detection, neutralization, diagnostic, and therapeutic studies.