Cell Purification Kit
Cell Purification Kit - Related Videos
17:25
RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit
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Cited by 1 •
2007
04:41
Magnetic-Activated Cell Sorting: A Method to Isolate c-Kit Positive Cells
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2023
This video describes the technique of isolating c-kit positive cells from the bone marrow of a mouse. In the protocol, we will isolate c-kit positive cells from a transgenic mouse to validate c-Fos and Dusp1 alone or together as drug targets in leukemia.
11:52
Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies
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Cited by 8 •
2007
Natural killer cells are a small population of lymphocytes. Here we show how to isolate these cells from human blood by negative selection, using a kit from StemCell Technologies. The cells obtained are viable and untouched by antibodies, and therefore ready to be used for a number of procedures.
08:16
Plasmid Purification
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2023
Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study. In this video, a step-by-step generalized procedure is described for how to perform plasmid purification.
14:26
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
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Cited by 59 •
2016
This paper presents a sensitive method called Circle-Seq for purifying extrachromosomal circular DNA (eccDNA). The method encompasses column purification, removal of remaining linear chromosomal DNA, rolling-circle amplification and high-throughput sequencing. Circle-Seq is applicable to genome-scale screening of eukaryotic eccDNA and studying genome instability and copy-number variation.
FAQs
Antibodies provide the recognition step: they bind surface markers associated with the population of interest. Magnetic particles or another separation matrix then supplies a physical way to retain cells linked through that binding, while other cells can be removed during washing. This coupling of biological specificity and physical separation helps enrich the selected population for downstream analysis.
Surface markers determine which cells are recognized, so marker choice directly affects the population that is retained and the biological conclusions drawn from it. In immunology, this is important when separating leukocytes or lymphocyte subsets from mixed samples. A well-matched marker strategy reduces cellular heterogeneity, making later measurements more attributable to the population under investigation.
Washing is not merely a handling step: it removes cells and sample material that are not sufficiently associated with the antibody-particle or matrix system. Effective washing therefore lowers background from unwanted cells and improves sample consistency. These effects matter when purified material is compared across conditions, because variable contamination can obscure differences in cellular composition or function.
A typical workflow begins with a complex biological sample, applies antibodies directed at the relevant surface markers, and uses magnetic particles or another separation matrix to create selective retention. Washing then removes unwanted material before the enriched population is taken forward. The resulting preparation can be analyzed by flow cytometry or used in culture, functional assays, or molecular analysis.
It is useful when mixed samples contain more cellular background than an assay can interpret reliably. Purifying leukocytes, lymphocyte subsets, or infected-cell populations can make flow-cytometric, culture-based, functional, and molecular measurements more focused, particularly when comparing host responses, pathogen interactions, or treatment effects.
Purified cells allow investigators to examine cellular composition and function in a less heterogeneous preparation, helping connect particular cell populations with host responses or pathogen interactions. The same preparation can also support assessment of treatment effects, while reduced background improves confidence that observed changes reflect the cells under study.