Journal
/
Developmental Biology
/
Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

DOI:
10.3791/56021-v

•

07:26 min

•

September 14, 2017

•

, ,
1Department of Biomedical Engineering,University of Arkansas

Chapters

  • 00:05Title
  • 00:50Cord Blood Endothelial Progenitor Cell Isolation
  • 03:34Endothelial Progenitor Cells Expansion
  • 04:58Results: Representative Cord Blood Endothelial Progenitor Cell Analyses
  • 06:21Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to isolate endothelial progenitor cells from umbilical cord blood. Some of the applications include using these cells as a biomarker for identifying patients with cardiovascular risk, treating ischemic diseases, and creating tissue-engineered vascular and heart valve constructs.

Tags

Keywords: Endothelial Progenitor CellsUmbilical Cord BloodVascular GraftsNeovascularizationCardiovascular DiseaseDensity Gradient CentrifugationMononuclear CellsEndothelial Cell Colonies

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56021?article_type=v

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
check_url/56021?article_type=v

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
check_url/56021?article_type=v

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
check_url/56021?article_type=v

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
check_url/56021?article_type=v

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/56021?article_type=v

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
check_url/56021?article_type=v

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
check_url/56021?article_type=v

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/56021?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
check_url/56021?article_type=v

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Read Article