Cre-loxp Genome Editing
Cre-loxp Genome Editing - Related Videos
01:59
Cre-LoxP Mediated Induction of Cerebral Cavernous Malformations in a Mouse Model
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2025
In this video, a genetically modified mouse pup carrying the Ccm2 gene flanked by loxP sites and Cre recombinase fused to an estrogen receptor is used to induce cerebral cavernous malformations (CCMs). The administration of 4-hydroxytamoxifen activates Cre in endothelial cells, leading to the deletion of Ccm2 and the formation of CCMs in the brain.
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
03:12
CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model
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2025
This video describes a procedure for target gene knockout using a tamoxifen-inducible Cre-recombinase system following intraperitoneal injection of tamoxifen in a mouse model.
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
08:51
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
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Cited by 30 •
2016
Transgenic manipulations and genome editing are critical for functionally testing the roles of genes and cis-regulatory elements. Here a detailed microinjection protocol for the generation of genomic modifications (including Tol2-mediated fluorescent reporter transgene constructs, TALENs, and CRISPRs) is presented for the emergent model fish, the threespine stickleback.
FAQs
The arrangement of paired loxP sequences determines what happens to intervening DNA. In one orientation, Cre-mediated recombination excises the segment; in another, it inverts it, while differing arrangements can support translocation. This makes sequence orientation a central design variable when researchers want to remove, reverse, or reposition genetic material in an experimental model.
Tissue-specific Cre expression confines recombination to selected cell or tissue contexts, whereas inducible expression adds control over timing. This matters in immunology because gene activity can be examined during a chosen biological window rather than across the entire organism. The resulting design helps connect a gene’s action to particular immune-cell processes or stages.
Conditional deletion can expose effects that a whole-organism disruption may hide or confound. Limiting the genetic change to a defined context helps researchers ask whether a phenotype arises from the targeted cells themselves or from broader systemic consequences. This comparison is especially useful when interpreting altered immune development, signaling, pathogen susceptibility, or host responses.
An experiment begins by identifying the gene or genomic segment whose function is being tested, then placing paired loxP sites so Cre can alter that segment in the intended context. Researchers next use tissue-specific or inducible Cre expression to control where or when the rearrangement occurs, and examine resulting immune or infection-related phenotypes.
By deleting a gene in a controlled cellular or temporal context, researchers can examine its contribution to immune-cell development, signaling, and host responses. The resulting phenotype provides functional evidence about the gene’s role rather than only showing that the gene is present. This approach is therefore useful for connecting genetic changes with defined immunological outcomes.
These models can test whether gene activity in selected immune contexts changes pathogen susceptibility or host responses. Because the alteration can be limited rather than organism-wide, resulting disease phenotypes can be interpreted alongside the targeted immune context. This supports disease-model development and analysis of how immune regulation contributes to infection outcomes.