Dendritic Cell Assay
Dendritic Cell Assay - Related Videos
11:39
T Cells Capture Bacteria by Transinfection from Dendritic Cells
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Cited by 2 •
2016
Here a protocol is presented to measure bacterial capture by CD4+ T cells which occurs during antigen presentation via transinfection from pre-infected dendritic cells (DC). We show how to perform the necessary steps: isolation of primary cells, infection of DC, DC/T cell conjugate formation, and measurement of bacterial T cell transfection.
05:16
A Vaccine-Pulsed Dendritic Cell-Based In Vitro Assay to Induce Lymphocyte Proliferation
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2025
This video demonstrates a monocyte-derived dendritic cell, MoDC-based assay to measure the immunogenicity of commercially available vaccines. MoDC assays investigate dendritic cell responses to antigens, pathogens, or treatments and can provide insights into immune responses, vaccine development, and immunotherapy research.
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
02:57
Bone Marrow-Derived Dendritic Cells Generation: A Method to Generate Dendritic Cells from Mouse Bone Marrow
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2023
This video describes a protocol for the generation of dendritic cells--key antigen-presenting cells that activate T cells--from the bone marrow of an adult mouse.
07:14
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
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Cited by 10 •
2016
An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.
FAQs
The assay follows how dendritic cells respond after encountering an antigen, pathogen-associated stimulus, or test compound. Changes in maturation markers and cytokine production indicate early sensing and activation, while antigen presentation and interactions with T cells show how that response may initiate adaptive immunity. Examining these stages together helps distinguish immediate cellular activation from downstream immune coordination.
Each readout captures a different aspect of dendritic cell activity. Maturation markers indicate changes associated with functional activation, cytokines reflect signaling output, and antigen presentation assesses the cell’s capacity to communicate antigen-related information. Measuring these outcomes together provides a broader profile than relying on one marker and can show whether a stimulus alters several connected functions.
A stimulus may activate, suppress, or otherwise alter dendritic cell function, and these different outcomes have distinct implications for immune regulation. Comparing response patterns helps researchers examine how dendritic cells contribute to inflammation or respond to infection-related signals. The results also clarify whether a test compound changes immune initiation rather than affecting only one isolated cellular readout.
Researchers first expose dendritic cells to an antigen, pathogen-associated stimulus, or test compound under in vitro conditions. They then evaluate selected outcomes, such as maturation markers, cytokine production, antigen presentation, or interaction with T cells. The workflow can be organized around one or several readouts, depending on whether the study focuses on activation, immune communication, or functional alteration.
Dendritic cell assays can help examine whether vaccine-related materials or immunotherapeutic approaches stimulate dendritic cell functions linked to immune initiation. Researchers may assess maturation, cytokine production, antigen presentation, or T-cell interaction to characterize the resulting response. These measurements provide experimental evidence about how a candidate influences the cellular connection between antigen detection and adaptive immune activity.
For immunotoxicity studies, researchers can expose dendritic cells to a test compound and determine whether it activates, suppresses, or alters their function. Readouts such as maturation markers, cytokines, antigen presentation, and T-cell interaction help identify changes across multiple immune processes. This approach is relevant when a compound’s potential effects on immune regulation need to be characterized in vitro.