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Isolation of Mouse Lung Dendritic Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Mouse Lung Dendritic Cells

Isolation of Mouse Lung Dendritic Cells

DOI:
10.3791/3563-v

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09:04 min

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November 22, 2011

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1Pathobiological Sciences,Louisiana State University

Chapters

  • 00:05Title
  • 01:20Lung Perfusion and Single Cell Suspension
  • 03:48Alternative Protocol for Obtaining a Single Cell Suspension
  • 04:26Magnetic Isolation and CD11c+ Cell Enrichment
  • 05:45Conventional Dendritic Cell Isolation
  • 06:46Combining Magnetic Bead Isolation and Flow Cytometric Cell Sorting Results in an Highly Purified Lung Conventional Dendritic Cell Population
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.

Tags

IsolationMouseLung Dendritic CellsDC SubsetsConventional DCPlasmacytoid DCIFN-producing Killer DCCD11cMHC-IIPulmonary MacrophagesSpecific MarkerIsolation Of CDC

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