Eae Mouse Model
Eae Mouse Model - Related Videos
02:27
Bioluminescence Imaging in an EAE Mouse Model
0 Views •
2025
Source: Schmitz, K., et al., Bioluminescence and Near-infrared Imaging of Optic Neuritis and Brain Inflammation in the EAE Model of Multiple Sclerosis in Mice. J. Vis. Exp. (2017).This video demonstrates the method for visualizing and monitoring Experimental Autoimmune Encephalomyelitis or EAE-induced inflammation in the optic nerve using bioluminescence imaging. It includes positioning the mouse, reagent injection, and capturing sequential images to monitor changes in bioluminescence over time.
02:26
Determining Blood-Brain Barrier Disruption Using Fluorescent Dyes in an EAE Mouse Model
0 Views •
2025
This video demonstrates a procedure for assessing blood-brain barrier disruption in an EAE mouse model. Fluorescent dyes of different sizes are injected retro-orbitally and circulate through the brain's blood vessels. The smaller dye leaks through gaps into the brain tissue, while the larger dye enters only if the disruption is severe, correlating with the extent of barrier damage.
08:05
Bioluminescence and Near-infrared Imaging of Optic Neuritis and Brain Inflammation in the EAE Model of Multiple Sclerosis in Mice
0 Views •
Cited by 4 •
2017
We show a technique for in vivo live bioluminescence and near-infrared imaging of optic neuritis and encephalitis in the experimental autoimmune encephalomyelitis (EAE) model for multiple sclerosis in SJL/J mice.
08:38
Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge
0 Views •
Cited by 1 •
2012
Certain mouse strains are able to resist induction of experimental autoimmune encephalomyelitis (EAE) with myelin basic protein. Described here is a simple immunization protocol that reverses the unresponsiveness and induces paralytic disease in several typical EAE resistant mouse stains.
09:24
Systemic Injection of Neural Stem/Progenitor Cells in Mice with Chronic EAE
0 Views •
Cited by 20 •
2014
The transplantation of neural stem/progenitor cells (NPCs) holds great promises in regenerative neurology. The systemic delivery of NPCs has turned into effective, low invasive, and therapeutically very efficacious protocol to deliver stem cells in the brain and spinal cord of rodents and nonhuman primates affected by experimental chronic inflammatory damage of the central nervous system.
FAQs
Myelin-derived antigen provides the immune target, while adjuvant supports activation of an autoimmune response in susceptible mice. Pertussis toxin is often included as part of this induction strategy. Together, these components promote activation of autoreactive T cells, creating an experimental setting in which researchers can examine immune-driven inflammation and demyelination in the central nervous system.
After activation, autoreactive T cells cross the blood-brain barrier and release inflammatory cytokines. Their entry and signaling help recruit and organize inflammatory responses within the central nervous system, linking peripheral immune activation to local tissue injury. This mechanism makes the model useful for studying leukocyte trafficking, barrier disruption, and the progression of neuroinflammation.
Clinical signs such as ascending paralysis provide an observable measure of disease development, but tissue and immune analyses add mechanistic detail. Researchers can examine leukocyte trafficking, blood-brain barrier disruption, inflammatory activity, demyelination, and tissue damage. Combining these outcomes helps connect clinical progression with the underlying cellular and pathological changes in the central nervous system.
A typical induction workflow uses susceptible mice that are immunized with a myelin-derived antigen formulated in an adjuvant, often alongside pertussis toxin. The animals are then monitored for clinical signs, while immune and tissue responses are analyzed. This sequence allows investigators to relate the initiating immune stimulus to CNS inflammation, demyelination, and neurological impairment.
Researchers may select this model when they need an in vivo system for examining immune-mediated CNS inflammation, demyelination, or autoimmune mechanisms relevant to multiple sclerosis. It also supports evaluation of immunomodulatory therapies and analysis of how immune cells interact with the blood-brain barrier. Its value comes from connecting immune events with clinical and tissue-level outcomes.
The model can be used to assess how infections or microbial stimuli influence central nervous system inflammation. Investigators compare the resulting immune and tissue responses with the autoimmune response produced by induction alone, focusing on changes in leukocyte trafficking, inflammatory activity, barrier disruption, or tissue damage. This provides context for studying interactions between host defense and autoimmune inflammation.