Ex Vivo Generation
Ex Vivo Generation - Related Videos
08:44
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
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Cited by 7 •
2013
Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.
09:44
Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo
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Cited by 56 •
2019
The goal of this article is to outline the steps required for the generation of fibrils from monomeric alpha-synuclein, subsequent quality control, and use of the preformed fibrils in vivo.
02:13
Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen
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2025
The video demonstrates the generation of an ex vivo ovine skin model infected with Staphylococcus aureus. This entails treating a lamb's forehead skin biopsy section with antibiotics, establishing a wound bed, positioning it on a permeable membrane in a multiwell plate with antibiotic-free medium, and establishing bacterial infection in the skin wound.
03:21
Murine Tail Lymphedema Model: An In Vivo Technique to Generate Sustained Lymphedema in Murine Tail
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2025
This video describes an in vivo technique to generate murine tail lymphedema, an abnormal fluid accumulation in the interstitial tissue space leading to swelling in the tail. The model is applied to study mechanisms and therapy for lymphedema.
04:42
An Ex Vivo Technique to Generate Tumor-Specific Chimeric Antigen Receptor T Cells
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2025
This video demonstrates a technique for the ex vivo generation of tumor antigen-specific chimeric antigen receptor (CAR) T cells. Retroviral vectors carrying transgenic RNA are mixed with spleen cells in a medium supplemented with interleukin-2 to promote T-cell survival and proliferation. The mixture is then transferred to a plate coated with recombinant human fibronectin fragments and centrifuged to facilitate the fusion of the virus with the cell, allowing the RNA to integrate into the host...
FAQs
Cell fate is shaped by the signals supplied during culture. Defined media, cytokines, growth factors, antigens, and other regulatory cues can be selected to support proliferation, differentiation, or expansion of an isolated primary-cell or precursor population. Changing these inputs lets investigators examine how particular signals influence immune-cell development and generate populations suited to a specific experimental question.
Ex vivo generation provides a controlled comparison that whole-animal experiments alone may not offer. Because investigators can regulate culture conditions and compare cellular responses directly, they can focus on mechanisms without relying solely on whole-animal experiments. This does not replace biological context, but it creates a more defined setting for testing how immune cells develop or respond to infection-related stimuli.
A phenotype assessment shows which cellular characteristics are present, whereas a functional assessment tests how those cells respond in the experimental system. Considering both measures helps determine whether culture conditions produced the intended immune-cell population and whether it behaves in a relevant way for infection, vaccine, or therapeutic studies.
A typical workflow starts with isolation of primary cells or precursor populations, followed by culture in defined media supplemented with selected cytokines, growth factors, antigens, or other signals. After the cells proliferate, differentiate, or expand under those conditions, investigators evaluate their phenotype and function. This sequence links culture inputs with measurable immune-cell outcomes.
These populations can be used to investigate host-pathogen interactions, immune-cell development, vaccine responses, and therapeutic strategies. Their value comes from connecting a defined cell state with a controlled experimental stimulus, allowing investigators to compare cellular mechanisms and responses to infection. The same general approach therefore supports both basic studies of immunity and evaluation of intervention-oriented questions.
Researchers can generate an immune-cell population, expose it to infection-related conditions, and then compare phenotype or function across cultures. This helps separate effects associated with cellular development from effects associated with pathogen-related stimulation, while retaining a system suitable for examining host-pathogen interactions. In immunology and infection, that separation strengthens mechanistic interpretation of cellular responses.