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Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo

Generation of Alpha-Synuclein Preformed Fibrils from Monomers and Use In Vivo

DOI:
10.3791/59758-v

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09:44 min

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June 02, 2019

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, , , , , , ,
1Department of Translational Science and Molecular Medicine,Michigan State University, 2The Michael J. Fox Foundation for Parkinson’s Research, 3Neuroscience Program,Michigan State University, 4Center for Neurodegenerative Disease Research, Department of Pathology and Laboratory Medicine,University of Pennsylvania Perelman School of Medicine, 5Center for Neurodegeneration and Experimental Therapeutics,University of Alabama at Birmingham, 6Mercy Health Hauenstein Neuroscience Medical Center

Chapters

  • 00:04Title
  • 01:13Formation of α-synuclein Preformed Fibrils from Monomers
  • 02:20Sonication of α-synuclein Preformed Fibrils
  • 04:37Preparation of Custom Glass Needle Syringes for Stereotactic Injections
  • 07:18Results: Analysis of the Generated Alpha-Synuclein Preformed Fibrils
  • 09:22Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this article is to outline the steps required for the generation of fibrils from monomeric alpha-synuclein, subsequent quality control, and use of the preformed fibrils in vivo.

Tags

Alpha-synucleinPreformed FibrilsParkinson’s DiseaseSynucleinopathyMonomersIn VivoCell CultureSonicationCentrifugationProtocol

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