Glycolytic Metabolism
Glycolytic Metabolism - Related Videos
06:47
Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
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Cited by 103 •
2015
Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.
01:24
Other Glycolytic Pathways
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2025
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
08:28
Metabolic Labeling
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2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
The pathway first uses ATP to activate glucose, preparing it for subsequent reactions and cleavage into three-carbon molecules. Later reactions generate ATP and NADH from those intermediates. This organization allows cells to spend energy initially and then recover energy while also producing metabolic intermediates that can support cellular activities.
NAD+ regeneration allows glycolytic reactions to continue when oxygen is limited or energy demand is high. Cells can reduce pyruvate to lactate, thereby restoring NAD+ from NADH. This maintains the pathway’s capacity to process glucose and generate ATP under conditions in which continued glycolytic activity is especially important.
Activation can increase glycolytic flux, meaning the rate or extent of glucose processing through the pathway. This shift helps immune cells address rapid energy requirements while supplying metabolic intermediates for biosynthetic needs. Examining flux therefore connects metabolic behavior with the functional demands imposed by an immune response.
In infection, host glucose metabolism may be altered by the presence or activity of pathogens, while some pathogens may rely on glycolysis themselves. These competing or interacting metabolic demands can change glucose use and glycolytic behavior. Studying such shifts helps relate metabolism to both pathogen activity and host immune responses.
Measurements of glycolytic shifts can show how cellular metabolism changes during immune activation or infection. Interpreting these changes alongside the biological condition may clarify whether altered glucose processing accompanies an immune response, reflects pathogen-associated activity, or signals a disease mechanism. The resulting information can guide investigation of metabolic therapeutic targets.
Researchers can examine changes in glycolytic flux to connect altered glucose processing with immune responses and infection-related disease mechanisms. Comparing metabolic behavior across relevant conditions may identify processes that support cellular activation or pathogen activity. Such findings can highlight metabolic steps or dependencies for further evaluation as potential therapeutic targets.