Ipsc-derived Macrophages
Ipsc-derived Macrophages - Related Videos
10:07
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
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Cited by 326 •
2013
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
05:06
Three-Dimensional Thymic Culture System to Generate iPSC-Derived Thymic Emigrants
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2025
This video demonstrates a protocol for generating functional antigen-specific T cells from iPSC-derived immature T cells using a three-dimensional thymic culture system. Co-culturing the iPSC-derived immature T cells with endogenous lymphocyte-depleted mice fetal thymic lobes resulted in successful maturation of the T cells to form CD8αβ+ MHC class I+ iPSC-derived thymic emigrants (iTEs).
07:06
Bone Marrow-derived Macrophage Production
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Cited by 205 •
2013
Macrophages have long been recognized as a critical component of the innate and adaptive immune responses. The recent explosion of knowledge pertaining to evolutionary, genetic, and biochemical aspects of the interaction between macrophages and microbes has renewed scientific attention to macrophages. This article describes a method to differentiate macrophages from mouse bone marrow.
06:23
Microglial Phagocytosis Assay: An In Vitro Assay to Establish Microglial Phagocytosis Model for Neuroblastoma Cells Using iPSC Macrophages
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2023
In this video, we perform the microglial phagocytosis assay for neuroblastoma using induced pluripotent stem cells. The assay helps assess the impairment of microglial function in neurodegenerative diseases.
07:53
Generating a Blood-Brain Barrier Chip Using iPSC-derived Precursor Cells
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2025
This video showcases creating a blood-brain barrier chip using iPSC-derived precursor cells. It involves seeding neural progenitor cells and brain microvascular endothelial cells in separate channels with a porous membrane. By flowing differentiation media, the endothelial cells form tight junctions resembling blood vessels, and the neural cells mature into various brain cell types, establishing a functional blood-brain barrier on the chip.
FAQs
Staged culture conditions provide the timing and environmental control needed to move cells from pluripotency through macrophage progenitors toward mature phagocytes. This staged progression matters because researchers can examine whether pathogen recognition, phagocytosis, cytokine release, or inflammatory responses change with cellular maturation. It therefore connects differentiation state with the innate immune behavior measured in experiments.
Macrophage progenitors serve as an intermediate developmental stage rather than the final experimental population. Their presence allows the differentiation process to be followed before mature phagocytes are obtained, helping investigators organize assays around defined cellular states. This distinction is useful when interpreting immune measurements, because progenitors and mature cells represent different points in the controlled culture sequence.
Researchers can expose these cells to defined experimental conditions and assess several innate immune outputs, including pathogen recognition, phagocytosis, cytokine release, and inflammatory responses. Considering these readouts together gives a broader view of host-pathogen interactions than relying on a single measurement. The same framework also supports investigations of disease mechanisms and therapeutic screening.
An experimental workflow begins with reprogramming mature cells to pluripotency, followed by staged culture conditions that direct differentiation into macrophage progenitors and then mature phagocytes. Researchers can subsequently place the generated cells in defined assays and measure recognition, phagocytosis, cytokine release, or inflammation. This sequence links cell production to functional testing in immunology and infection studies.
Their renewable source and scalable production can provide cells for repeated experiments, while their human origin preserves relevance to human immune biology. Using them can also reduce reliance on primary tissue samples. These features are particularly valuable when studies require defined conditions or comparisons across multiple experimental settings involving pathogen responses, inflammatory activity, or therapeutic effects.
The model is suited to host-pathogen interaction studies, disease-mechanism investigations, therapeutic screening, and analysis of patient-specific immune phenotypes. Because cells can be generated from iPSCs and examined under controlled conditions, investigators can connect a human cellular background with measurable innate immune outputs. In immunology and infection, this supports testing how defined contexts influence recognition, phagocytosis, cytokine release, and inflammation.