Lesion Measurement
Lesion Measurement - Related Videos
03:00
Measuring Drug Binding to Caseum from Tuberculosis Lesions via the Rapid Equilibrium Dialysis Method
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2025
Source: Sarathy, J. P. et al. An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions. J. Vis. Exp. (2017)This video demonstrates an in vitro assay for measuring drug binding to caseum from tuberculosis lesions using rapid equilibrium dialysis. It outlines the steps for preparing caseum suspensions, performing dialysis, and calculating the unbound drug fraction to predict drug penetration into caseum.
09:31
Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
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Cited by 1 •
2013
We measured the tension release in an axon that was partially lesioned with a laser dissector by simultaneous force spectroscopy measurement performed on an optically-trapped probe adhered to the membrane of the axon. The developed experimental protocol evaluates the axon adhesion to the culture substrate.
09:22
Sectioning Mammary Gland Whole Mounts for Lesion Identification
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Cited by 13 •
2017
We developed a method to successfully remove, process, section, and stain, for histopathological evaluation, mammary tissue that had originally been fixed on slides as whole mounts. This method may promote the collection and evaluation of mammary gland whole mounts in reproductive and developmental test guideline studies.
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
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2019
This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.
05:51
A Human Ex Vivo Atherosclerotic Plaque Model to Study Lesion Biology
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Cited by 13 •
2014
Atherosclerosis is a chronic inflammatory process. This manuscript illustrates an easy to use ex vivo model to investigate fresh carotid or coronary artery plaques. The ex vivo model allows for the investigation of potential substances on the inflammatory milieu in human atherosclerotic lesions and results can be analyzed by various methods.
FAQs
Reliable lesion measurement depends on applying the same boundary rules and recording approach across observations. Investigators first identify the visible extent of the lesion, then quantify a selected feature such as dimensions, area, or volume. This standardization reduces variation caused by inconsistent interpretation, making differences over time more likely to reflect biological change rather than measurement technique.
Dimensions, area, and volume do not represent identical summaries of pathology. Dimensions capture selected linear extent, while area or volume can represent the broader spatial scale of abnormal tissue when those measures are recorded. Selecting and maintaining one defined outcome helps investigators compare lesions consistently and interpret changes in disease progression or treatment response.
Consistent time points make lesion measurements useful for tracking change. Recording observations according to a planned schedule allows investigators to compare the lesion across stages of disease or treatment rather than relying on isolated impressions. The resulting pattern can indicate progression or response, while also linking visible tissue change with host inflammatory or pathogen-associated damage.
These approaches provide different ways to quantify defined lesion features. Calipers can be used when direct dimensions are the selected outcome, while imaging or digital analysis can be used when investigators record dimensions, area, or volume from captured tissue changes. The appropriate choice is the one that matches the planned measure and can be applied consistently.
A basic workflow begins by identifying the lesion boundary, selecting the measurement type, and recording the result at consistent time points. Investigators then repeat that process across subjects or observations using the same definitions and tool category. Keeping these steps aligned produces comparable data for evaluating changes in tissue damage, swelling, or abnormal growth.
In infection and immunology studies, lesion measurements help translate visible pathology into data that can be compared across disease progression or treatment response. Investigators can use the measurements to examine pathogen-associated damage and host inflammatory responses, then relate changes in lesion appearance to therapeutic efficacy. This supports quantitative evaluation of infection models without relying only on descriptive observation.