Lung Fibrosis Monitoring
Lung Fibrosis Monitoring - Related Videos
02:48
Preparation of Artificial Sputum Medium to Emulate Cystic Fibrosis Lung Sputum Ex Vivo
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2025
Source: Vieira, J., et al. Design and Development of a Model to Study the Effect of Supplemental Oxygen on the Cystic Fibrosis Airway Microbiome. J. Vis. Exp. (2021).This video demonstrates the preparation of an artificial sputum medium that mimics the viscosity, nutrient composition, and pH of cystic fibrosis lung sputum. The resulting medium provides a physiologically relevant platform for ex vivo studies.
07:46
Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
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Cited by 159 •
2012
Current diagnostic antimicrobial susceptibility testing relies on the planktonic growth of isolates in nutrient rich, aerobic conditions. Here, we employ an alternative artificial sputum medium to study antimicrobial susceptibility of Pseudomonas aeruginosa biofilms under both aerobic and microaerophilic conditions more representative of the cystic fibrosis lung.
01:47
Lung Capacity
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2019
The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures. There are four defined lung volume measures: tidal volume, inspiratory reserve volume, expiratory reserve volume, and residual volume. Tidal volume is the amount of air inhaled and exhaled in a normal breath. Inspiratory...
09:49
Measurement of the Pressure-volume Curve in Mouse Lungs
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Cited by 47 •
2015
Here we present a protocol to simply and reliably measure the lung pressure-volume curve in mice, showing that it is sufficiently sensitive to detect phenotypic parenchymal changes in two common lung pathologies, pulmonary fibrosis and emphysema. This metric provides a means to quantify the lung’s structural changes with developing pathology.
05:15
Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung
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Cited by 22 •
2013
The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular disease and for developing therapeutic intervention. Here a noninvasive intratracheal intubation method that can directly deliver exogenous materials to mouse lungs is presented.
FAQs
A single observation shows the patient’s status at one point, but repeated assessments reveal whether lung function and structural changes are stable, worsening, or improving. Tracking trends in symptoms, oxygenation, pulmonary function, and imaging helps clinicians judge disease activity and progression more reliably, while also supporting interpretation of treatment response over time.
Forced vital capacity provides information about changing lung function, while diffusing capacity helps assess how effectively the lungs transfer gases. Following both measurements can show functional decline that may not be captured by symptoms alone. Their serial patterns contribute to evaluating progression and determining whether a therapeutic strategy is producing a meaningful response.
The monitoring record combines symptoms, oxygen assessment, pulmonary function trends, and high-resolution computed tomography findings. Considering these measures together can help researchers and clinicians assess whether ongoing changes are more consistent with persistent inflammatory or infectious injury or with progressive remodeling of lung tissue. This distinction is important when interpreting disease activity and treatment effects.
High-resolution computed tomography provides visual information about the architecture of fibrotic change, complementing functional tests and clinical observations. Comparing scans over time can show whether structural abnormalities are changing alongside pulmonary measurements. This combined perspective helps relate visible remodeling to functional decline and supports a more complete assessment than either imaging or physiology alone.
A monitoring workflow may include symptom review, oxygen assessment, pulmonary function testing, and high-resolution computed tomography when structural evaluation is needed. Forced vital capacity and diffusing capacity are especially important functional measures. Repeating these assessments creates a time-based record that can be used to examine progression, disease activity, and response to treatment.
Serial clinical, functional, and structural measurements provide outcomes for evaluating immunomodulatory therapies and antifibrotic strategies. Researchers can compare trends in symptoms, oxygenation, pulmonary function, and fibrotic architecture rather than relying on one measurement. In immunology and infection research, the same framework also helps assess whether treatment affects persistent injury, remodeling, or patient risk.