
Darla Goeres
Montana State University
Dr. Darla Goeres has over twenty years of experience researching biofilm bacteria in industrial systems. She has studied biofilms found in beer draught lines, Danish district heating distribution pipes, anaerobic biofilms in soured oil fields, biofilms in recreational water systems, and evaluated various treatment strategies for killing, removing and/or preventing biofilm formation in the built environment. Dr. Goeres leads the Standardized Biofilm Methods Laboratory at the Center for Biofilm Engineering at Montana State University. The mission of this laboratory is the development and validation of quantitative standard methods for growing, treating, sampling and analyzing biofilm bacteria. As part of her work in this area, she is the Chair of American Society for Testing and Materials (ASTM) Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and facilitated the acceptance of the first approved standard methods for biofilm bacteria. Her goal is to promote collaboration among the various stakeholders interested in biofilm methods.

Erin Gloag
Ohio State University
Dr. Erin Gloag completed her PhD at the University of Technology Sydney, under the supervision of Professor Cynthia Whitchurch and Dr. Lynne Turnbull. Her PhD research focused on understanding microbial collective behaviors during biofilm expansion. She moved to the United States to perform her postdoctoral research in Professor Daniel Wozniak’s lab at the Ohio State University. Her research, performed in collaboration with Professors Vaughn Cooper and Paul Stoodley, has focused on how Pseudomonas aeruginosa biofilms persist during infection. Dr. Gloag is now a Research Scientist in Professor Wozniak’s lab.

Daniel Wozniak
Ohio State University
Professor Daniel Wozniak completed his PhD at the Ohio State University in Professor Darrell Galloway’s lab and his postdoctoral research at the University of Tennessee in Professor Dennis Ohman’s lab. He moved to Wake Forest University School of Medicine as an Assistant Professor. During his time at Wake Forest, he was promoted to full Professor. He then moved to back to the Ohio State University, where he is now the Vice Chair of the Department of Microbial Infection and Immunity and co-director of the Cure CF Columbus research and development program. Professor Wozniak is a world leading expert on microbial pathogenesis and biofilm biology, where he has over 4 decades of research experience in these fields. He has published over 100 research manuscripts, and his work has been cited over 12,000 times.
Biofilms have traditionally been studied on either glass or plastic surfaces. From these studies pervading the field is the understanding that biofilms display a lifecycle whereby planktonic cells attach to a surface, develop into mature mushroom-shaped structures, after which, individual planktonic cells are released that go on to reinitiate the lifecycle. Due to the dominance of these studies in the field, the mushroom structure is largely considered the quintessential biofilm morphology. However, observations of biofilms in situ, in the natural environment, industry, and medical settings, reveal that this is not the case. For example, in biofilm-associated chronic infections, biofilms are routinely observed as smaller aggregated communities rather than the structured films observed on glass and plastic surfaces. Furthermore, in the context of chronic infections, there are few models that recapitulate the pathology of the human disease. These disparities have impacted advances in understanding biofilm-associated chronic infections, compared to acute infections that are caused by planktonic cells.
This biofilm methods collection aims to collate models of infection that analyze host-pathogen and microbial interactions away from traditional glass or plastic surfaces. The goal of this collection is to offer a resource for both new and existing researchers in the biofilm field that provides in-depth details of various biofilm models of infection, and a discussion of the advantages and limitations of each. This will allow researchers to select models that are appropriate for the research questions and analysis they are exploring in relation to biofilm-associated infections.
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Cited by 2
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2021
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Real time assessment of antimicrobial tolerance in bacterial aggregates
Alexa Gannon1,
Sophie Darch*1
1University of South Florida
Modification of the MBEC biofilm assay using a “JEKMagTech” - stainless steel magnetic pins technology.
Haifeng Ji1,
Garth Ehrlich2,
Jarosław Król*2,
Donald Hall Jr.3
1Department of Chemistry, Drexel University,
2Center for Advanced Microbial Processing, Center for Surgical Infections and Biofilms, Department of Microbiology and Biotechnology, Drexel University College of Medicine,
3Department of Chemistry, Drexel University. Center for Advanced Microbial Processing, Center for Surgical Infections and Biofilms, Department of Microbiology and Biotechnology, Drexel University College of Medicine
Bead movement based computational framework for analysis of biofilm material properties
Bettina Buttaro*1,
Gillian Queisser*1
1Temple University