Metabolic Selection
Metabolic Selection - Related Videos
02:53
Identification of Recombinant Vaccinia Virus-Infected Cells Using Metabolic and Fluorescent Selection
0 Views •
2026
Source: Marzook, N. B., et al., Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins. J. Vis. Exp. (2014)This video demonstrates the generation of recombinant vaccinia viruses using plasmid-mediated homologous recombination in infected epithelial cells. Fluorescent and metabolic markers enable the selection and identification of viruses expressing tagged proteins.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
0 Views •
Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
00:52
What is Metabolism?
0 Views •
2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
10:19
An Introduction to Cell Metabolism
0 Views •
2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
08:28
Metabolic Labeling
0 Views •
2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
The available nutrient acts as a functional test of cellular metabolism. Cells able to use that nutrient can continue surviving and proliferating, while cells lacking the required pathway are eliminated or outcompeted. Changing the nutrient environment therefore changes which metabolic capabilities are favored and can reveal differences among cells within an initially mixed population.
Metabolic inhibitors create a defined blockade that challenges a particular cellular capability. Cells that tolerate the blockade or retain an alternative pathway can persist, whereas sensitive cells decline. This design helps investigators associate survival with pathway activity or resistance to metabolic stress, rather than relying only on differences observed under unrestricted growth conditions.
Enrichment reflects both survival and relative proliferation. A cell may persist under selective conditions yet remain underrepresented if another cell type grows more efficiently, while a modest growth advantage can produce strong enrichment over time. Interpreting results therefore requires attention to the selective environment and to how competing populations respond under the same conditions.
Immune cells in different states may depend on different metabolic pathways or respond differently to nutrient limitation and metabolic blockade. Applying defined selective conditions can enrich populations associated with particular metabolic capabilities, allowing researchers to compare their survival and expansion. These patterns provide context for understanding how cellular metabolism contributes to immune responses during infection.
A study begins by choosing a nutrient condition or metabolic inhibitor that tests the capability of interest, then exposing a mixed cell or microorganism population to that defined environment. Investigators monitor which populations survive or proliferate and compare the enriched outcome with the starting population. The selected groups can then be examined for their relevant metabolic traits.
This strategy is useful when researchers need to examine how pathogens adapt to nutrient-limited environments or how host cells respond to metabolic stress during infection. Enriched populations can highlight pathways that support survival in the host context, helping clarify host-pathogen interactions and identifying metabolic processes that may warrant antimicrobial or immunomodulatory investigation.
Enrichment can indicate that a metabolic capability is associated with survival under the applied condition, but it does not by itself establish every mechanism responsible. Comparing the selected population with the original population can reveal candidate pathways linked to persistence, adaptation, or immune-cell state. These findings can guide further analysis of potential intervention targets.