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Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
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JoVE Journal Neuroscience
Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

DOI:
10.3791/51276-v

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14:11 min

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May 04, 2014

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, , , , , , , ,
1Center for Neuroscience, Swammerdam Institute for Life Sciences,University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Section Molecular Cytology, Swammerdam Institute for Life Sciences,University of Amsterdam

Chapters

  • 00:05Title
  • 02:05Coverslip Preparation
  • 02:48Dissection of the Hippocampi
  • 03:58Cell Dissociation and Plating
  • 05:35Rat Hippocampal Primary Neuron Transfection using Lipofectamine
  • 07:23Immunostaining Hippocampal Primary Neurons
  • 08:54Dendritic Spine Imaging using Structure Illumination Microscopy
  • 12:12Results: Dendrites and Dendritic Spines Imaged with SIM and Confocal Microscopy
  • 13:22Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail.

Tags

Keywords: Dendritic SpinesRat Primary Hippocampal NeuronsStructured Illumination Microscopy (SIM)Super-resolution MicroscopyNeuronal ConnectivitySynaptic PlasticityNeuron CultureFluorescent Protein3D Reconstruction

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