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Neuroscience

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Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
 

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

Article doi: 10.3791/51276
May 4th, 2014

Summary May 4th, 2014

This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail.

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