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Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
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JoVE Journal Neuroscience
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation

Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation

DOI:
10.3791/3109-v

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09:50 min

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October 06, 2011

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, , ,
1Department of Anatomy and Neurobiology, School of Medicine,University of California, Irvine, 2Department of Biomedical Engineering, School of Engineering,University of California, Irvine

Chapters

  • 00:05Title
  • 01:32Brain Slice Preparation
  • 02:17Equipment Startup
  • 03:24Whole Cell Patch-clamp Recording
  • 05:52Laser Scanning Photostimulation
  • 07:44Representative Results for Electrophysiological Recording and Photostimulation Mapping

Summary

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Automatic Translation

This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.

Tags

Inhibitory NeuronsNeuronal CircuitsLaser Scanning PhotostimulationCortical FunctionSubtypesImmunochemical PropertiesMorphological PropertiesPhysiological PropertiesLocal Circuit ConnectionsExcitatory Synaptic InputInhibitory Synaptic InputLaser Scanning Photostimulation (LSPS)Electrical StimulationGlutamate Puff StimulationMapping ResolutionCortical Circuit AnalysisWhole-cell Patch Clamping

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