Mouse Paw Processing
Mouse Paw Processing - Related Videos
09:14
Paw-Dragging: a Novel, Sensitive Analysis of the Mouse Cylinder Test
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Cited by 53 •
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Classical forelimb asymmetry analysis of the cylinder test is routinely used to assess behavioural deficits in rats following brain injury or stroke; however, it fails to detect consistent deficits in mice. This study demonstrates that quantifying paw-dragging behaviour is a more sensitive analysis of brain injury in mice.
11:03
A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
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A cryogenic pulverization method to process murine paws using a liquid nitrogen freezer mill was developed to improve the yield and quality of RNA or protein extracted from the tissues and enable the analysis of molecular profiles associated with inflammatory responses.
07:49
Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus
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Electronic von Frey measurements are an effective and improved alternative to classical von Frey filaments, as they allow rapid and precise measurement of changes in rodent mechanical withdrawal thresholds to continuously applied pressure stimuli before and after an inflammatory event.
04:44
Processing of a Mouse Brain for Downstream Analysis
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This video demonstrates a detailed step-by-step protocol for preparing frozen mouse brain tissue sections for downstream analyses.
08:24
Experimental Assessment of Mouse Sociability Using an Automated Image Processing Approach
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This protocol describes a method to quantify mouse sociability. Mice are videotaped as they move and interact in a special cage. Movie processing allows for the automated quantification of sociability with excellent accuracy and reliability.
FAQs
Mechanical disruption or homogenization breaks the collected paw tissue into a recoverable sample, helping release cells, proteins, nucleic acids, and infectious agents for downstream analysis. The extent and consistency of disruption matter because uneven processing can affect how much biological material is available for measurement. Standardized disruption therefore supports more reliable comparisons of inflammation, infection, and immune responses.
Timing and consistent handling help preserve biological signals present in the paw at collection. Delays or differences between samples may influence measurements of immune-cell infiltration, cytokines, pathogen burden, or inflammatory pathology. Applying the same collection and processing approach across experimental groups improves comparability and makes observed differences more likely to reflect the experimental condition rather than sample preparation.
Processed paw tissue can provide several complementary readouts. Flow cytometry can examine recovered immune cells, culture can assess infectious agents, microscopy can evaluate inflammatory pathology, and molecular assays can measure nucleic-acid-based signals. Protein-focused analyses can assess cytokine production. Selecting among these approaches depends on whether the study emphasizes cellular infiltration, pathogen burden, inflammatory mediators, or tissue changes.
A typical workflow begins by collecting the paw tissue, removing unwanted material, and mechanically disrupting or homogenizing the sample. The processed material is then directed toward an assay suited to the study question, such as flow cytometry, culture, microscopy, or a molecular analysis. Careful, consistent handling throughout the workflow helps preserve measurable biological features.
The intended measurement should guide how the processed tissue is used. Samples examined by flow cytometry should support recovery of cells, whereas culture is suited to investigating infectious agents. Molecular assays use nucleic-acid-containing material, and microscopy focuses on tissue pathology. Protein-related analyses can address cytokine production. This alignment helps ensure that processing produces information relevant to the experimental objective.
The mouse paw provides a localized site for examining inflammation, infection, and immune activity in the extremities. Processing allows investigators to compare experimental groups using measures such as immune-cell infiltration, pathogen burden, cytokine production, and inflammatory pathology. These localized readouts can connect tissue-level changes with the immune or infectious response being investigated.