Etheno Adduct Analysis
Etheno Adduct Analysis - Related Videos
11:46
Single Molecule Analysis of Laser Localized Psoralen Adducts
0 Views •
2017
Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.
07:52
Removal of Branched and Cyclic Compounds by Urea Adduction for Uk'37 Paleothermometry
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst As mentioned in previous videos, the product of an organic solvent extraction, a total lipid extract (TLE), is often a complex mixture of hundreds, if not thousands, of different compounds. The researcher is often only interested in a handful of compounds. In the case of our two organic paleothermometers (Uk'37 and MBT/CBT), the interest is in only 6 compounds (2 alkenones and 4 isoprenoidal glycerol dialkyl glycerol...
09:33
Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
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Cited by 3 •
2018
Evaluating the potency of environmental chemicals and drugs, to be enzymatically bioactivated to intermediates generating covalent DNA adducts, is an important field in the development of cancer and its treatment. Methods are described for compound activation to form DNA adducts, as well as techniques for their detection and quantification.
10:10
An Overview of Alkenone Biomarker Analysis for Paleothermometry
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst Throughout this series of videos, natural samples were extracted and purified in search of organic compounds, called biomarkers, that can relate information on climates and environments of the past. One of the samples analyzed was sediment. Sediments accumulate over geologic time in basins, depressions in the Earth into which sediment flows through the action of fluid (water or air), movement, and gravity. Two main types...
15:04
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
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Cited by 46 •
2009
This article describes a novel protocol and reagent set designed for sensitive measurement of neurotoxic effects of compounds and treatments on co-cultures of neurons and astrocytes using high content analysis. Results demonstrate that high content analysis represents an exciting novel technology for neurotoxicity assessment.
FAQs
Inflammation can generate reactive aldehydes through lipid peroxidation, creating electrophilic compounds that react with DNA nucleobases. The resulting chemical modifications provide a molecular link between inflammatory conditions and genotoxic stress. Measuring these lesions therefore helps determine whether an immune or infection-associated response is accompanied by DNA damage rather than relying only on broader indicators of inflammation.
Immunochemical methods detect modified nucleosides through antibody-based recognition, whereas liquid chromatography coupled with mass spectrometry separates analytes and measures their mass-related signals. These approaches provide complementary analytical strategies: immunochemical assays can support detection in biological samples, while LC-MS offers measurement linked to chromatographic separation and comparison with authentic standards.
Authentic standards provide reference compounds for identifying and quantifying the modified nucleosides released from DNA or biological samples. Comparing sample measurements with these known materials strengthens confidence that the detected signal corresponds to the intended etheno-modified product. This is particularly important when results are used for biomarker development or exposure assessment.
A typical workflow begins with isolating DNA or preparing a biological sample, followed by enzymatic treatment that releases modified nucleosides. The released products are then examined using an immunochemical assay or liquid chromatography coupled with mass spectrometry. Including suitable reference standards supports interpretation and allows the measured adduct signal to be related to the original sample.
In immunology and infection studies, etheno adduct measurements can indicate whether inflammatory responses or pathogen-associated damage are associated with oxidative and electrophilic stress. The results help connect immune or infectious processes with genotoxic effects at the molecular level. They may also support comparisons among biological conditions and the development of stress-related biomarkers.
Researchers can compare adduct measurements across samples or conditions before and after an antioxidant or anti-inflammatory intervention. A change in the measured lesion burden may provide evidence that the treatment influenced the oxidative or electrophilic stress associated with DNA modification. Such results can complement broader exposure or inflammation assessments when evaluating intervention effects.