Nasopharyngeal Sample Processing
Nasopharyngeal Sample Processing - Related Videos
12:21
Examining the Role of Nasopharyngeal-associated Lymphoreticular Tissue (NALT) in Mouse Responses to Vaccines
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Cited by 39 •
2012
Methods to examine contributions of the nasopharyngeal-associated lymphoreticular tissues (NALT) to nasal and systemic immune responses of mice to intranasal vaccines are described. We demonstrate a surgical procedure to establish a NALT-dependent mouse model and ex vivo cultures of extracted NALT.
01:29
Suctioning the Nasopharyngeal Airway
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2025
Nasopharyngeal suctioning is a procedure to remove secretions from the upper part of the respiratory tract that the patient cannot clear independently. It helps maintain airway patency and prevents complications such as aspiration pneumonia. Equipment Required Suction machine with adjustable pressure settings Sterile suction catheters (appropriate size for the patient) Sterile gloves Sterile saline or water (for irrigation or lubrication) Water-soluble lubricant Personal protective...
05:07
Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models
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Cited by 20 •
2018
The practical application and performance of methods for generation of representative tissue samples of porcine animal models for a broad spectrum of downstream analyses in biobank projects are demonstrated, including volumetry, systematic random sampling, and differential processing of tissue samples for qualitative and quantitative morphologic and molecular analyses types.
11:20
In Situ Visualization of the Phase Behavior of Oil Samples Under Refinery Process Conditions
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Cited by 1 •
2017
This article describes a setup and method for the in situ visualization of oil samples under a variety of temperature and pressure conditions that aim to emulate refining and upgrading processes. It is primarily used for studying isotropic and anisotropic media involved in the fouling behavior of petroleum feeds.
11:54
Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples
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Cited by 51 •
2018
This manuscript describes the use of nasal and bronchial absorption techniques, specifically using synthetic absorptive matrices (SAM) to sample the mucosal lining fluid (MLF) of the upper and lower airway. These methods provide better standardization and tolerability than existing respiratory sampling techniques.
FAQs
Mixing is important because it releases mucus, epithelial cells, leukocytes, and microorganisms from the swab into transport medium. This creates a more representative processed sample for downstream testing. In immunology and infection studies, the resulting material can support both pathogen detection and measurement of local host responses.
Centrifugation or clarification can remove or separate particulate material before analysis, helping prepare the specimen for assays that require a less particulate input. This step is not an endpoint by itself; its value depends on the intended measurement, because nucleic-acid extraction, antigen testing, and cellular or cytokine analysis may use different portions or characteristics of the processed material.
Consistent handling helps preserve sample integrity across specimens and reduces variation introduced during preparation. It also supports control of inhibitors and contamination, two factors that can interfere with reliable detection or interpretation. Standardized processing therefore makes results more comparable when researchers examine respiratory pathogens alongside local immune measurements.
A practical workflow moves from the swab in transport medium to mixing, controlled aliquoting, and, when appropriate, centrifugation or clarification. The prepared material is then directed toward nucleic-acid extraction, antigen testing, or cellular and cytokine analysis. Keeping these decisions explicit helps align the portion analyzed with the scientific question and limits avoidable variation between specimens.
The downstream assay should guide how processed material is handled and allocated. Nucleic-acid extraction supports pathogen genetic analysis, whereas antigen testing examines pathogen-associated antigen detection. Cellular and cytokine analyses instead focus on host immune responses. Matching processing choices to the assay helps prevent a single preparation strategy from obscuring the measurement of interest.
The approach supports diagnostic testing and respiratory-pathogen surveillance, while also serving research on mucosal immunity. In infection studies, processed specimens can be examined for pathogens together with local host responses; after vaccination, they can contribute to investigations of immune changes at the respiratory surface. This links laboratory handling to both clinical and research questions.