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Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

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Developmental Biology

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JoVE Journal Developmental Biology

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles

DOI:

10.3791/55904-v

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10:07 min

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May 26, 2017

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Abhijit Nirwane, Jyoti Gautam, Yao Yao

¹College of Pharmacy,University of Minnesota

Chapters

00:05Title
00:47Muscle Dissection and Single-cell Isolation
03:02Cell Sorting
07:03Post-sorting Analyses
08:22Results: Distinct Characteristics of Type I and Type II Pericytes
09:35Conclusion

Summary

Automatic Translation

This work describes a FACS-based protocol that allows for easy and simultaneous isolation of type I and type II pericytes from skeletal muscles.

Tags

Keywords: Pericyte Isolation Type I Pericyte Type II Pericyte Skeletal Muscle FACS Nestin-GFP Collagenase Trypsin-EDTA Red Blood Cell Lysis Cell Sorting Cell Suspension

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