Serum Sample Collection
Serum Sample Collection - Related Videos
08:23
Seawater Sampling and Collection
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Cited by 13 •
2009
This video documents methods for collecting coastal marine water samples and processing them for various downstream applications including biomass concentration, nucleic acid purification, cell abundance, nutrient and trace gas analyses.
09:32
Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach
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Cited by 2 •
2017
This protocol provides a method for the collection of mouse embryonic stem cell (mESC)-conditioned medium (mESC-CM) derived from serum (fetal bovine serum, FBS)- and feeder (mouse embryonic fibroblasts, MEFs)-free conditions for a cell-free approach. It may be applicable for the treatment of aging and aging-associated diseases.
09:13
Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice
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Cited by 49 •
2011
This article describes the procedures for conducting a basic postmortem examination of a mouse or rat, and the collection of basic organs, as well as more challenging sample types from for histological, microbiological, and PCR evaluation.
07:22
Standardized Method for Measuring Collection Efficiency from Wipe-sampling of Trace Explosives
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Cited by 7 •
2017
Optimized sampling protocols and the development of new wipe materials can be facilitated by standardized measurements of collection efficiency from wipe-sampling. Our approach for sampling trace explosives uses an automated device to control speed, force, and distance during wipe-sampling followed by extraction of collected explosives.
08:43
Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery
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Cited by 9 •
2017
Endothelial progenitor cells (EPCs) are crucially involved in the neovascularization of ischemic tissues. This method describes the isolation of human EPCs from peripheral blood, as well as the identification of their migratory potential against serum samples of cardiac surgical patients.
FAQs
Clotting creates the starting separation needed for serum analysis. As blood coagulates, a clot forms and cellular material becomes distinct from the liquid fraction that remains. This matters because the resulting serum can be examined for antibodies, antigens, and other immune or infection-related biomarkers without treating the original whole-blood mixture as the analytical specimen.
Centrifugation physically separates the liquid serum from the cells and clot after clotting has occurred. This step produces a more defined specimen for transfer into a labeled tube, reducing ambiguity about which fraction is being analyzed. In immunology and infection studies, that separation supports more consistent measurement of target biomarkers across samples.
Handling consistency affects whether measured biomarkers remain representative of the collected specimen. Using the same overall collection, separation, labeling, and storage approach helps preserve analyte stability and makes results more comparable between experiments or clinical studies. This is particularly important when immune measurements are compared across participants, study groups, or sampling points over time.
A basic workflow begins by collecting blood, allowing it to clot, and centrifuging the sample so the serum separates from cells and the clot. The serum is then transferred into a labeled tube and maintained under appropriate storage conditions. Each stage links the physical preparation of the specimen to its later use in immune or infection-related testing.
Serum samples can support measurements of antibodies, antigens, and other biomarkers associated with immune or infectious processes. These measurements provide material for serology, diagnostic testing, and broader immune profiling. Because the same type of specimen can be collected at multiple sampling points, serum also helps investigators examine changes in immune or infection-related responses over time.
Within immunology and infection research, serum collection is useful for vaccine evaluation and for monitoring responses over time, in addition to diagnostic and serological work. Its value comes from linking a standardized specimen-preparation process with assays that assess immune or infection-related markers. Careful handling strengthens comparisons among study samples and supports interpretation of changing responses.