Tissue Core Extraction
Tissue Core Extraction - Related Videos
08:30
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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Cited by 53 •
2016
This modified extraction protocol improves RNA and DNA yields from more precisely targeted regions of interest in histopathologic tissue blocks.
09:21
Sediment Core Sectioning and Extraction of Pore Waters under Anoxic Conditions
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Cited by 5 •
2016
A protocol for sectioning sediment cores and extracting pore waters under anoxic conditions in order to permit analysis of redox sensitive species in both solids and fluids is presented.
08:32
Extraction of Tissue Antigens for Functional Assays
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2012
A simple protocol for preparing extracts of human tissue to be used as a source of antigens in functional T-cell assays is described. This method allows T-cell responses to tissue-derived antigens to be measured in vitro.
02:10
The Nucleosome Core Particle
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2020
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins. The paradox Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
04:38
Isolation of Amyloid Fibrils from Brain Tissue Extract
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2025
This video demonstrates the protocol for isolating amyloid fibrils from mouse brain tissue extract using density gradient centrifugation and digestion of non-amyloid proteins.
FAQs
The sampling geometry is controlled by the hollow cutting needle or coring device. As it advances into tissue, its cutting edge shears out a defined column, preserving a cylindrical representation of the sampled site while limiting disruption to adjacent material. This controlled shape helps connect the specimen’s structural, cellular, and molecular findings to a specific region of disease.
Consistency in core size and sampling is important because tissue responses can vary across a diseased site. Comparable extraction allows investigators to compare specimens more reliably rather than attributing differences to uneven sampling. In immunology and infection studies, this supports interpretation of changes in immune-cell distribution, pathogen-associated tissue alterations, disease progression, or treatment effects.
Preserving the sampled location matters because immune responses and pathogen-associated changes are assessed within diseased tissue, not just as isolated measurements. A core retains a localized column that can be examined for structure, cellular distribution, and molecular features. This spatially anchored evidence helps investigators connect local tissue findings with disease progression and treatment effects.
After removal, the core may be stabilized, sectioned, or processed for a downstream assay, depending on the intended evidence. Stabilization and sectioning support structural and cellular examination, including histology and immunostaining. Processing for nucleic acid analysis provides molecular information. Matching preparation with the assay helps preserve the relevant readout from the same defined tissue region.
These samples support several complementary readouts: histology for tissue structure, immunostaining for cellular or tissue-associated patterns, and nucleic acid analysis for molecular information. They can also be used to assess immune-cell distribution and pathogen-associated changes. Considering these readouts together helps relate local tissue organization to immune activity and infection-related alterations.
It is particularly useful when investigators need information from a diseased site while limiting disruption to the surrounding specimen. The approach supports comparisons among specimens and can be applied when the study tracks local responses across disease progression or treatment effects. Its value is greatest when structural, cellular, or molecular findings must remain linked to the sampled location.