Tissue Culture Cells
Tissue Culture Cells - Related Videos
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
08:47
Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System
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Cited by 10 •
2015
Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
13:08
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture
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Cited by 80 •
2013
A method to process human mammary surgical discard material is described. Processed tissue, in the form of organoids, can be stored frozen indefinitely or placed in culture for long-term growth. This method enables experimental examination of normal human epithelial cell biology, and the effects of exogenous perturbations.
FAQs
Successful maintenance depends on coordinating several conditions rather than supplying nutrients alone. Sterile nutrient media supports cell survival and division, while appropriate temperature and gas exchange preserve the controlled environment. Researchers also select an attachment surface or suspension system according to how the cells are maintained. These variables determine whether cultures remain viable and can be expanded through passage.
Passage keeps a growing culture available for continued study by moving cells into fresh cultures. This step supports further multiplication rather than treating the original vessel as a permanent experiment. In infection and immunology work, continued passage helps provide cells for repeated investigations of pathogen effects, inflammatory signaling, or treatment responses under controlled conditions.
Researchers can examine pathogen entry, replication, inflammatory signaling, and cytotoxicity as distinct cellular outcomes. Using host-cell cultures creates a controlled setting for observing how infection changes cell behavior and triggers immune-related responses. These observations help connect events occurring at the cellular level with disease mechanisms without requiring every question to be addressed directly in an organism.
A basic workflow supplies sterile nutrient media, places cells under appropriate temperature and gas exchange, uses an attachment surface or suspension system, and transfers the cells into fresh cultures as they multiply. This sequence preserves the conditions needed for survival, division, and continued experimental use. The exact setup depends on the cell system being maintained.
Researchers turn to these cultures when they need controlled cellular testing and animal or human studies are impractical. The systems can be used to assess responses to vaccines or antimicrobial treatments, while also examining how pathogens affect host cells. This makes them useful for connecting treatment-related or infection-related cellular responses with broader disease mechanisms.
Maintaining comparable culture conditions allows investigators to interpret cellular responses more consistently across experiments. Reproducible systems can support repeated examination of pathogen effects, inflammatory signaling, cytotoxicity, or treatment responses. That consistency strengthens efforts to relate cellular mechanisms to disease outcomes and provides a controlled basis for comparing findings when animal or human studies are impractical.