Tumor Entrained Neutrophils
Tumor Entrained Neutrophils - Related Videos
04:21
Generating Anti-Angiogenic Tumor-Associated Neutrophils
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2025
This video demonstrates an assay to generate anti-angiogenic tumor-associated neutrophils (TANs). Upon taking a murine melanoma tumor containing pro-angiogenic TANs, the single cells are isolated using fluorescence-activated cell sorting (FACS) and treated with an inhibitor of nicotinamide phosphoribosyl transferase (NAMPT) to reduce the capacity to stimulate angiogenesis.
10:15
Isolation and Characterization of Neutrophils with Anti-Tumor Properties
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Cited by 46 •
2015
Neutrophils play an important role not only in host defense against invading microorganisms, but are also involved in the immune surveillance of tumor cells. Here, we describe techniques related to the isolation of neutrophils with anti-tumor properties and methods for monitoring anti-tumor neutrophil function in vitro and in vivo.
04:21
Isolation of Cytotoxic Neutrophils from Tumor-Bearing Mouse Blood
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2025
In this video, we demonstrate the isolation of cytotoxic high-density neutrophils and low-density neutrophils from the blood of a tumor-bearing mouse by discontinuous sucrose gradient centrifugation.
01:27
Air-entraining Agents
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2024
Air-entraining agents improve the durability and workability of concrete in climates with frequent freezing and thawing. These agents prevent cracks by introducing small air bubbles into the mix, creating spaces accommodating water expansion when temperatures drop. The air-entraining agents lower the surface tension of water, forming stable, small air bubbles. This method is more effective than having accidental large voids, as the intentional, smaller, and evenly distributed air voids improve...
04:32
Induction and Visualization of Neutrophil Extracellular Traps in Pre-Labeled Neutrophils
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2025
This video demonstrates the induction and visualization of neutrophil extracellular traps or NETs in pre-labeled neutrophils using patient serum containing anti-neutrophil cytoplasmic antibodies. Furthermore, using an impermeable DNA dye and fluorescence microscopy enables the visualization of NETs.
FAQs
Tumor-derived cytokines, growth factors, and chemokines can act as signals that alter neutrophil recruitment, activation, survival, and effector functions. In this setting, cells may acquire behavior distinct from circulating neutrophils, rather than simply increasing in number. This functional reprogramming helps explain how a tumor can redirect innate immune activity toward inflammation, immune suppression, and tumor progression.
The tumor microenvironment provides surrounding signals that influence how neutrophils behave after recruitment. These conditions can affect whether neutrophils contribute to inflammatory activity, immune suppression, extracellular trap formation, angiogenesis, or tumor-cell invasion. Examining neutrophils within this local context is therefore important because their effects cannot be understood from circulating-cell behavior alone.
Their functional state differs because tumor-associated signals can reshape several neutrophil properties at once, including recruitment, activation, survival, and effector activity. Circulating neutrophils provide a useful comparison for identifying these changes. This distinction helps investigators determine whether observed neutrophil activity reflects ordinary innate immune behavior or tumor-associated reprogramming linked to cancer progression.
Several altered effector activities may connect neutrophils with tumor progression. These include extracellular trap formation, inflammatory signaling, immune suppression, effects on angiogenesis, and promotion of tumor-cell invasion. Considering these activities together is important because tumor-entrained neutrophils may affect both the immune environment and the behavior of tumor cells, potentially supporting local growth or metastasis.
A study can examine how tumor-associated signals affect neutrophil recruitment, activation, survival, and effector functions. It can then relate those changes to inflammation, immune suppression, extracellular trap formation, angiogenesis, or tumor-cell invasion. This approach connects cellular behavior with tumor outcomes and helps distinguish a change in neutrophil presence from a change in neutrophil function.
They are relevant when researchers investigate how tumors reshape host defenses, particularly the behavior of innate immune cells. In an immunology context, the topic links environmental signaling with altered neutrophil activity and immune suppression. It also provides a framework for studying how cancer-associated changes in host defense may influence inflammation, invasion, and metastatic progression.
Characterizing tumor-entrained neutrophils may help identify biomarkers associated with neutrophil-mediated tumor activity. It may also guide treatments designed to interrupt signals or functions that support tumor growth and metastasis. The research value lies in connecting measurable neutrophil changes with cancer progression, while identifying immune processes that could be targeted without treating neutrophils as uniformly protective or harmful.