Amniotic Membrane Extract
Amniotic Membrane Extract - Related Videos
04:21
Lumican Extraction from Amniotic Membrane and Determination of its Storage Temperature
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2022
The present protocol describes the extraction of lumican from the amniotic membrane (AM) and their storing conditions as AM extract (AME) at -20 °C, 4 °C, and room temperature (RT) for 6, 12, 20, and 32 days to quantify its proteins and lumican concentration.
09:34
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Cited by 3 •
2017
This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.
10:00
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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Cited by 8 •
2019
This protocol describes the isolation of epithelial cells from different anatomical regions of the human amniotic membrane to determine their heterogeneity and functional properties for possible application in clinical and physiopathological models.
07:36
Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats
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Cited by 20 •
2015
Encephalopathy of prematurity encompasses the central nervous system abnormalities associated with injury from preterm birth. This report describes a clinically relevant rat model of in utero transient systemic hypoxia-ischemia and intra-amniotic lipopolysaccharide administration (LPS) that mimics chorioamnionitis, and the related impact of infectious stimuli and placental underperfusion on CNS development.
04:15
Extracting Host Cell Lysate from Infected Host Cells Cultured in a Permeable Membrane System
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2025
This video demonstrates a permeable membrane insert system to investigate the impact of bacterial toxins on host cells in a controlled and isolated manner. This approach allows for the selective exposure of the cells to the toxins while maintaining a physical separation that mimics the natural host-pathogen interaction.
FAQs
They may influence injured or inflamed tissue through interactions between preserved soluble molecules and local cells or tissue structures. The proposed response includes changes in inflammatory signaling alongside support for cell attachment, cell migration, and tissue repair. Because these effects depend on the preparation’s retained components, biological activity cannot be assumed to be identical across products.
Extracellular-matrix components can support cell attachment and migration, processes relevant to organizing repair at an injured site. In this context, the matrix is part of the biologic material whose interaction with tissue may contribute to repair, rather than simply a residual structural element. This rationale supports investigation in wound-care and ocular-surface settings.
Composition may vary with both the tissue source and the way the material is processed. Those differences can alter which soluble molecules and extracellular-matrix components remain in the final preparation, making product-to-product comparisons difficult. Characterization and standardization are therefore important for linking a preparation’s composition with its safety profile and therapeutic effectiveness.
Modulation of inflammatory signaling is one part of the proposed activity in injured or inflamed tissue. The preparation is investigated not only for supporting cell attachment and migration, but also for influencing signaling associated with inflammation while tissue repair proceeds. This combined biological rationale helps explain its investigation in lesions and ocular-surface damage.
A general workflow starts with processing the innermost fetal membrane, but the resulting composition depends on how that processing is performed. Evaluation therefore requires characterization of retained soluble molecules and extracellular-matrix components, attention to tissue source, and efforts toward standardization. Clinical evidence remains necessary to assess whether a preparation is safe and therapeutically effective.
Clinical and translational interest centers on wound care, ophthalmology, and regenerative applications. Examples include difficult-to-heal lesions and ocular-surface damage, where supporting cell attachment, migration, inflammatory modulation, and repair may be relevant. These applications must be interpreted alongside preparation-specific composition and clinical evidence because source and processing can influence safety and therapeutic effectiveness.