Antegrade Endoscopic Vein Harvesting
Antegrade Endoscopic Vein Harvesting - Related Videos
00:09
Synergizing Antegrade Endoscopic with Bridging Vein Harvesting for Improvement of Great Saphenous Vein Graft Quality from the Lower Leg
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2019
Presented here is a protocol for antegrade endoscopic vein harvesting from the lower leg, which can safely be introduced in routine coronary artery bypass grafting. Vein grafts present excellent graft quality following this standardized protocol with positioning of the legs, minimally invasive access to the vein, and antegrade endoscopic vein harvesting.
03:42
Jugular Vein Graft Harvest From a Rabbit Model: A Surgical Procedure to Excise a Segment of Rabbit Jugular Vein for Ex Vivo Studies
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2025
In this video, we demonstrate a procedure for surgically harvesting a segment of the jugular vein from a rabbit model. The resected segment can be used as an autologous vein graft to study intimal hyperplasia that develops due to arterial blood pressure.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
06:53
Selective Harvesting of Marginating-hepatic Leukocytes
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Cited by 1 •
2016
Marginating-hepatic leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe a method for selective harvesting of this specific hepatic cell population, through forced perfusion of the liver of rats or mice. Marginating-hepatic leukocytes seem critical in determining susceptibility to hepatic-related diseases and metastases.
FAQs
Advancing in the direction of blood flow helps the surgeon follow the vein’s natural course within the tissue plane. The endoscope provides visual guidance while dissection proceeds through small incisions, allowing the operator to identify and divide side branches without treating the conduit as an exposed open wound. This supports preservation of a usable graft.
The main distinction is the access route. Endoscopic harvesting uses small skin incisions and visualization through an endoscope, whereas open harvesting requires a more extensive incision along the vein’s course. The minimally invasive approach can reduce incision size and wound-related complications while still providing a conduit for later vascular reconstruction.
Side branches must be divided so the main venous segment can be separated from surrounding tissue and removed as a continuous conduit. Endoscopic visualization helps the surgeon identify these branches during dissection. Careful branch management contributes to graft preparation by preserving the segment intended for coronary or peripheral vascular reconstruction.
The procedure begins with small skin incisions that allow access to the vein and placement of an endoscope. Under endoscopic guidance, the surgeon dissects in the natural tissue plane while advancing along the direction of blood flow. Side branches are divided, and the selected vein segment is then preserved as a graft.
A harvested vein serves as a conduit when surgeons need to reconstruct or bypass a vessel. The overview identifies two major settings: coronary artery bypass surgery and peripheral vascular bypass procedures. In both contexts, the preserved segment provides graft material that can be prepared for the planned vascular reconstruction.
The technique supports two linked outcomes: preparation of a usable vascular conduit and potentially easier recovery from the harvesting incision. Because access occurs through smaller incisions than open harvesting, the approach can reduce wound-related complications. These features are relevant when vein removal accompanies major coronary or peripheral vascular surgery.