Cardiac Graft Function
Cardiac Graft Function - Related Videos
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
06:22
Modified Langendorff Perfusion for Extended Perfusion Times of Rodent Cardiac Grafts
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Cited by 2 •
2024
This article demonstrates the feasibility of achieving longer perfusion times (4 h) of murine cardiac grafts without function loss by employing lower (30-35 mmHg) than physiological (60-80 mmHg) perfusion pressures during Langendorff.
08:49
Cardiac Loading using Passive Left Atrial Pressurization and Passive Afterload for Graft Assessment
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Cited by 7 •
2024
The protocol describes a porcine ex vivo heart perfusion system in which direct loading of the left ventricle may serve as an assessment technique for graft health while simultaneously providing a holistic evaluation of graft function. A discussion of the system design and possible assessment metrics is also provided.
11:38
Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
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Cited by 36 •
2010
Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
FAQs
Performance depends on several interacting processes rather than contraction alone. Myocardial contractility drives ventricular ejection, while ventricular filling supplies the volume needed for effective output. Coronary perfusion supports the transplanted myocardium, and electrical rhythm coordinates contraction. Clinicians therefore interpret these factors together when judging how well the graft supports the recipient’s circulation.
Ischemia-reperfusion injury is an important influence on early graft performance because the myocardium is affected by periods of reduced oxygen supply and subsequent restoration of blood flow. Its effects are considered alongside contractility, filling, perfusion, and rhythm. This combined assessment helps clinicians recognize impaired function and evaluate how the graft recovers after transplantation.
Immune-mediated rejection and immunosuppressive therapy can both influence how a transplanted heart performs. A change in graft function therefore requires clinical interpretation within the broader transplant context rather than evaluation of contraction in isolation. Monitoring these factors supports recognition of complications, guides treatment decisions, and contributes to understanding of long-term transplant outcomes.
Strong contraction does not fully describe effective graft performance if ventricular filling is inadequate. Filling provides the volume that the ventricles can eject, while contractility determines how effectively they generate forward flow. Considering both measurements gives clinicians a more complete view of circulation through the graft and helps identify which aspect of performance may require attention.
Assessment combines complementary tools. Echocardiography evaluates cardiac performance, hemodynamic measurements provide information about circulatory function, and electrocardiography examines electrical rhythm. Cardiac biopsy is used when indicated, particularly as an additional assessment within transplant care. Together, these approaches help detect dysfunction, investigate complications, guide treatment, and follow recovery over time.
Serial assessment can help identify primary graft dysfunction and later complications, while also showing how the transplanted heart adapts to the recipient’s circulation. Findings support timely intervention and treatment selection. Over a longer period, the same monitoring framework helps clinicians evaluate transplant outcomes and improve understanding of recovery and graft performance.