Crosslinking Reaction
Crosslinking Reaction - Related Videos
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
03:06
Reaction Mechanisms
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2020
Chemical reactions often occur in a stepwise fashion, involving two or more distinct reactions taking place in a sequence. A balanced equation indicates the reacting species and the product species, but it reveals no details about how the reaction occurs at the molecular level. The reaction mechanism (or reaction path) provides details regarding the precise, step-by-step process by which a reaction occurs. For instance, the decomposition of ozone appears to follow a mechanism with two steps:
02:53
Determining Order of Reaction
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2020
Rate laws describe the relationship between the rate of a chemical reaction and the concentration of its reactants. In a rate law, the rate constant k and the reaction orders are determined experimentally by observing how the rate of reaction changes as the concentrations of the reactants are changed. A common experimental approach to the determination of rate laws is the method of initial rates. This method involves measuring reaction rates for multiple experimental trials carried out using...
FAQs
Crosslink density changes how closely polymer chains or biomolecules are connected. As connectivity rises, materials often become stiffer, swell less, dissolve less readily, and resist degradation more effectively. Lower density can preserve greater swelling and flexibility. These tradeoffs let researchers tune a material for its intended medical role.
Covalent connections generally provide a chemically joined network, whereas reversible interactions represent a different form of connectivity within the material. This distinction matters because interaction type can affect the resulting stiffness, swelling, solubility, and resistance to degradation. Comparing these outcomes helps researchers select a network design suited to a particular medical use.
Crosslinkers or activating conditions bring reactive functional groups into a state where connections can form between chains or biomolecules. Their role determines how molecular connectivity is introduced, while reaction chemistry influences the resulting material properties. Controlling these inputs helps researchers tailor networks rather than treating all crosslinked materials as physically equivalent.
Biocompatibility must be considered because a material intended for medicine needs to perform safely. Reaction chemistry and crosslink density can be evaluated together with compatibility when researchers design hydrogels or other biomaterials. This integrated view supports materials whose mechanical and degradation-related properties are useful for therapy, tissue repair, or cell culture.
Measurements of stiffness, swelling, solubility, and resistance to degradation show how network formation changes material behavior. Together, these properties help characterize whether a candidate material can maintain its intended form and remain suitable for a medical application. They also provide practical outcomes for comparing formulations with different crosslink densities.
Crosslinked hydrogels can serve as platforms for controlled drug delivery, tissue repair, and cell culture. Their network properties are important because changing molecular connectivity can alter stiffness, swelling, solubility, and degradation resistance. Researchers can therefore match hydrogel behavior to the intended application while considering reaction chemistry, crosslink density, and biocompatibility.
In corneal medicine, collagen crosslinking is used to strengthen corneal tissue. This application illustrates how modifying connectivity in a biomolecule can produce a medically relevant change in tissue properties rather than merely changing laboratory material behavior. Its therapeutic relevance depends on understanding the reaction chemistry and maintaining biocompatibility for safe use.