Ex Vivo Organ Perfusion
Ex Vivo Organ Perfusion - Related Videos
09:29
Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
0 Views •
Cited by 31 •
2014
Marginal grafts, such as fatty livers, grafts from older donors, or livers retrieved after cardiac death (DCD) tolerate conventional, cold static storage only poorly. We developed a novel model of subnormothermic ex vivo liver perfusion for preservation, assessment, and repair of marginal liver grafts prior to transplantation.
08:12
Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation
0 Views •
Cited by 43 •
2015
The severe organ shortage has resulted in increased use of marginal kidney grafts for transplantation. This has triggered interest in alternative storage methods, since marginal grafts especially tolerate cold storage poorly. The technique of normothermic ex vivo kidney perfusion (NEVKP) represents a novel preservation method for kidney grafts prior to transplantation.
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
0 Views •
Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
0 Views •
Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
0 Views •
2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
FAQs
The circuit supplies oxygen and nutrients through the organ’s blood vessels, allowing cells to continue metabolic activity outside the body. It also regulates temperature, pressure, and flow, which helps create controlled conditions for maintaining organ function. These controls are important because preservation depends not only on supplying fluid, but also on managing the physical environment during the procedure.
Temperature, pressure, and flow determine how closely the perfusion environment supports continued organ activity. Controlling these variables helps deliver the perfusion solution through the vascular system while maintaining conditions suitable for cellular metabolism. Their regulation also provides a standardized setting in which investigators can observe organ function and evaluate how the organ responds during preservation or treatment.
Conventional cold storage primarily preserves an organ during a limited preservation interval, whereas Ex Vivo Organ Perfusion can maintain controlled circulation through the organ outside the body. This difference may extend preservation beyond cold storage and allows functional assessment before transplantation. The approach therefore adds an opportunity to evaluate organ condition rather than relying only on storage preservation.
The isolated organ is connected to a perfusion circuit through its blood vessels, and a perfusion solution is circulated through the organ. The system then controls temperature, pressure, and flow while delivering oxygen and nutrients. During this period, organ function can be maintained and assessed, creating a controlled workflow for preservation, evaluation, or further conditioning.
Functional testing during perfusion can help assess how an organ performs while it is supported outside the body. Because the circuit maintains circulation and controlled conditions, clinicians and researchers can evaluate organ function before transplantation rather than depending solely on its initial preservation status. This information can support decisions about medical use and help characterize transplant readiness.
In transplantation, the technique can help preserve organs for longer than conventional cold storage, assess their function, and condition or repair organs that might otherwise be unsuitable. In research, it provides a platform for studying organ physiology, injury, and transplant outcomes. Its value lies in combining organ support with an opportunity to observe and influence the organ before use.