Facs Staining
Facs Staining - Related Videos
06:04
Murine Dermal Fibroblast Isolation by FACS
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Cited by 28 •
2016
Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
17:07
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The overall function of the immune system is to defend the body against infectious...
03:12
May-Grunwald Giemsa Staining: A Method to Stain Bone Marrow Cells
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2023
This video describes the technique of staining bone marrow cells using May- Grünwald Giemsa Staining. In the protocol, we will stain the murine myeloid leukemia cells, C149 cells, to analyze the morphology and differential counting.
11:04
Adoptive Cell Transfer: Introducing Donor Mouse Splenocytes to a Host Mouse and Assessing Success via FACS
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2023
Source: Meunier Sylvain1,2,3, Perchet Thibaut1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France Adoptive cell transfer is a method for introducing cells into a patient or study organism...
11:33
Microscopy and Staining: Gram, Capsule, and Endospore Staining
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2023
Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1 1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. To observe these properties, one...
FAQs
Antibodies bind specific proteins associated with individual cells and carry fluorescent labels that can be excited by the flow cytometer’s lasers. The resulting emitted fluorescence is measured as each cell passes through the instrument. Signal intensity and marker patterns allow researchers to distinguish cellular populations according to the proteins they express.
Controls help determine whether detected fluorescence represents a true marker-associated signal rather than an ambiguous measurement. Because multiple fluorescent labels may produce overlapping emission signals, spectral overlap must also be corrected during analysis. Together, these measures improve confidence that observed marker patterns accurately reflect the cell sample.
Multiparameter analysis measures several cellular markers simultaneously rather than evaluating one marker in isolation. Combining these signals can separate distinct cell populations and provide information about their functional states. This broader profile is especially useful when related populations share some proteins but differ in their overall marker pattern.
The workflow begins with a prepared cell suspension and incubation with an antibody panel selected for the proteins of interest. Cells are then washed to remove unbound reagents before entering the flow cytometer. Laser excitation and fluorescence detection generate measurements for individual cells, which are subsequently interpreted using the selected markers and controls.
By measuring proteins on or within individual cells, FACS staining can distinguish immune-cell populations within a mixed suspension. Multiparameter measurements add detail about marker combinations and functional states. This makes the technique useful for characterizing immune profiles rather than relying only on the total number of cells present.
In clinical and research medicine, the method supports classification of leukemia and lymphoma by examining cellular marker patterns. It also contributes to infection monitoring and the assessment of treatment responses. Because measurements are made across individual cells and multiple markers, results can reveal changes in cellular populations or states associated with disease or therapy.