Glutamine Uptake
Glutamine Uptake - Related Videos
10:23
Glutamine Flux Imaging Using Genetically Encoded Sensors
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Cited by 7 •
2014
This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.
04:04
Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids
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2025
This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.
08:01
LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
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Cited by 6 •
2017
Here we present a step-by-step protocol of the long-length electrostatic repulsion-hydrophilic interaction chromatography-tandem mass spectrometry (LERLIC-MS/MS) method. This is a novel methodology that enables for the first time quantification and characterization of the glutamine and asparagine deamidation isoforms by shotgun proteomics.
03:27
Preparing Cells to Analyze Intracellular Uptake and Activity of an Antiviral Agent
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2026
Source: Allaire, A., et al. Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus. J. Vis. Exp. (2015).The video demonstrates the procedure for preparing cells to assess the intracellular uptake and activity of an antiviral agent against the hepatitis C virus.
08:03
Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
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Cited by 14 •
2017
In this method, human primary muscle cells are cultured in vitro to obtain differentiated myotubes and glucose uptake rates are measured. We provide a detailed protocol to quantify rates in basal and insulin-stimulated states using radiolabeled [3H] 2-deoxy-D-Glucose.
FAQs
Transporter activity determines how strongly cells acquire glutamine. ASCT2 and SNAT proteins represent sodium-dependent systems, so their function is linked to ion gradients across the cell membrane. Extracellular glutamine availability also limits the amount that can enter. Together, transporter activity, the surrounding glutamine concentration, and gradient conditions help explain why uptake differs among cells or physiological states.
Sodium dependence gives some uptake pathways a direct connection to membrane ion conditions. When the relevant gradient changes, transporter-mediated entry may change even if extracellular glutamine remains available. This matters because uptake is not controlled by glutamine concentration alone. Interpreting an uptake result therefore requires considering both external amino acid supply and the transport environment that permits cellular entry.
An increase in uptake does not by itself identify a single cellular outcome. Once inside, glutamine can support metabolism, biosynthesis, and nitrogen balance, so elevated transport may reflect increased demand for one or several functions. Conversely, reduced uptake can indicate altered transporter activity, limited availability, or changed ion-gradient conditions. These alternatives should be distinguished when interpreting metabolic data.
Measuring glutamine uptake can reveal how cells respond to changing nutrient demand or disease-related metabolic adaptation. In medical research, the result is most informative when linked to cellular context, such as growth, immune activation, or tissue repair. Uptake measurements can therefore serve as a readout of transport behavior and nutrient use, rather than a complete explanation of cell function by themselves.
Researchers examine glutamine uptake in tumors because altered nutrient acquisition may accompany increased cellular growth and metabolic demand. Comparing uptake across disease states or treatment conditions can help identify whether tumor cells depend on this transport process. That dependence may point to a metabolic vulnerability, making uptake relevant to studies that evaluate therapies designed to target cancer metabolism.
In immune-cell studies, changes in glutamine entry can be considered alongside activation because glutamine supports cellular metabolism and biosynthesis. The same principle applies to tissue repair, where altered nutrient demand may accompany regenerative activity. Examining uptake in these settings helps connect membrane transport with broader cellular responses, while avoiding the assumption that one uptake value alone proves activation or successful repair.