Hemoglobin-based Perfusate
Hemoglobin-based Perfusate - Related Videos
01:24
Hemoglobin
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2024
Hemoglobin is a globular protein made up of four subunits. Two of these subunits are alpha chains, and the other two are beta chains. Each subunit contains a molecule of heme, which has an iron atom and can bind to oxygen. When an oxygen molecule binds to one heme group, it changes the shape of hemoglobin, making it easier for the other heme groups to bind oxygen as well. When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
06:37
Staphylococcus aureus Growth using Human Hemoglobin as an Iron Source
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Cited by 31 •
2013
Here we describe a growth assay for Staphylococcus aureus using hemoglobin as the sole source of available nutrient iron. This assay establishes the role of bacterial factors involved in hemoglobin-derived iron acquisition.
09:47
Exploring Alternative Perfusion Solutions Using Next-Generation Polymerized Hemoglobin-Based Oxygen Carriers in a Model of Rat Ex Vivo Lung Perfusion
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Cited by 3 •
2024
Here, we describe the application of a polymerized human hemoglobin (PolyhHb)-based oxygen carrier as a perfusate and the protocol in which this perfusion solution can be tested in a model of rat ex vivo lung perfusion.
04:51
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
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2025
This video demonstrates a method to generate a rat model of neonatal intraventricular hemorrhage. In this procedure, an anesthetized rat pup is injected with hemoglobin into the lateral ventricle of the brain. The hemoglobin causes oxidative stress and releases heme, simulating intraventricular hemorrhage. The resulting damage to brain tissues, driven by reactive oxygen species and inflammatory cytokines, leads to ventricular enlargement, a common consequence of intraventricular hemorrhage.
05:18
A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering
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Cited by 1 •
2016
A photo-thermal angular light scattering (PT-AS) sensor enables the rapid and chemical-free hemoglobin assay of nanoliter-scale blood samples. Here, details of the PT-AS setup and a measurement protocol for the hemoglobin concentration in blood are provided. Representative results for anemic blood samples are also presented.
FAQs
The central mechanism is reversible oxygen loading and unloading. In oxygenated conditions, hemoglobin binds oxygen; as oxygen levels fall locally within the perfused tissue, it releases that oxygen. This enables the circulating fluid to support cellular respiration through the organ’s vascular system rather than merely bathing tissue in an oxygen-poor solution.
Compared with conventional crystalloid fluids, the key distinction is oxygen-carrying capacity. Crystalloids carry little oxygen, whereas a hemoglobin-containing formulation can transport oxygen and release it where local levels decrease. This difference matters when perfusion aims not only to circulate fluid, but also to support cellular respiration during organ maintenance outside the body.
Performance depends on more than the presence of hemoglobin. The formulation must provide effective oxygen transport, remain compatible with the organ’s vascular system, and help protect tissue function. Its design may use purified, modified, or encapsulated hemoglobin, making these properties important considerations when selecting a formulation for circulation and extended support.
During ex vivo perfusion, the solution is circulated through an isolated organ or tissue’s vascular system under oxygenated conditions. Hemoglobin loads oxygen in that environment, then releases it as local oxygen levels fall. The perfusate thereby supports a workflow that can maintain the organ, permit assessment, and contribute to preservation before transplantation.
Researchers may consider a hemoglobin-based perfusate when oxygen delivery is needed beyond what conventional crystalloid perfusion provides. Its medical relevance lies in combining circulation with oxygen transport during ex vivo organ maintenance and assessment. This makes it an alternative for preservation workflows in which tissue function must be supported over an extended period before transplantation.
Ex vivo perfusion with this fluid can help maintain cellular respiration while an isolated organ or tissue remains in circulation, supporting assessment of tissue function during preservation. Interpretation depends on whether oxygen transport, vascular compatibility, and tissue protection are adequate. These criteria connect perfusate performance with the quality of organ support before transplantation.