Mouse Lung Graft
Mouse Lung Graft - Related Videos
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
13:04
Generation and Grafting of Tissue-engineered Vessels in a Mouse Model
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Cited by 13 •
2015
Here, we present a protocol to generate tissue engineered vessel grafts that are functional for grafting into mice by double seeding partially induced pluripotent stem cell (PiPSC) - derived smooth muscle cells and PiPSC - derived endothelial cells on a decellularized vessel scaffold bioreactor.
03:35
A Technique for Human Skin Grafting in a Mouse Model
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2025
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.
12:39
Implantation of Inferior Vena Cava Interposition Graft in Mouse Model
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Cited by 24 •
2014
To improve our knowledge of cellular and molecular neotissue formation, a murine model of the TEVG was recently developed. The grafts were implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. This model achieves similar results to those achieved in our clinical investigation, but over a far shortened time-course.
FAQs
After implantation, the recipient’s immune system can detect donor antigens, meaning biological markers recognized as foreign. That recognition may be associated with graft acceptance, inflammation, or rejection. By controlling donor and recipient conditions, investigators can use the model to compare these immune outcomes and examine transplant immunology in a focused setting.
Reconnecting the airway and blood supply is central because the graft must be surgically integrated with the recipient as part of the transplant model. These connections establish the conditions needed to investigate graft function alongside immune responses, inflammation, and other consequences of implantation. Their inclusion also makes the experimental setup relevant to pulmonary transplantation.
Ischemia-reperfusion injury is a distinct research focus because the mouse lung graft permits investigators to examine this transplant-associated problem under controlled experimental conditions. Studying it alongside graft function and immune responses helps separate related components of post-transplant biology. The model therefore supports testing how potential therapeutic strategies affect injury and overall graft outcomes.
At a high level, the procedure includes selecting a donor and recipient, surgically implanting a section or whole lung, and reconnecting the graft’s airway and blood supply. Subsequent observation allows investigators to examine acceptance, inflammation, rejection, ischemia-reperfusion injury, and graft function. This workflow links technical implantation with biological assessment.
It can provide information about whether a graft is associated with acceptance, inflammation, or rejection, while also supporting assessment of graft function and ischemia-reperfusion injury. These outcomes let researchers connect an intervention or experimental condition with both immune and graft-related consequences. Such controlled comparisons are useful for evaluating strategies intended to improve transplantation results.
Because the system permits controlled experimental analysis, it allows researchers to investigate transplant immunology, graft function, ischemia-reperfusion injury, and tissue repair in a pulmonary setting. Findings from these studies may inform human lung transplantation and regenerative medicine, particularly when investigators evaluate therapeutic strategies designed to improve outcomes after transplantation.