Hypothermic Cardioplegia
Hypothermic Cardioplegia - Related Videos
08:39
Establishment of Deep Hypothermic Circulatory Arrest in Rats
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Cited by 5 •
2022
This protocol presents the establishment of deep hypothermic circulatory arrest in rats, which can be applied to investigate systemic inflammatory response syndrome, ischemia/reperfusion injury, oxidative stress, neuroinflammation, etc.
08:30
Murine Isolated Heart Model of Myocardial Stunning Associated with Cardioplegic Arrest
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Cited by 3 •
2015
The goal of this protocol to assess myocardial stunning following ischemic cardioplegic arrest in rodents.
03:37
Porcine Heart Excision: A Protocol to Extract Porcine Heart for Cardiac Transplant
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2025
In this video, we describe a step-by-step protocol to extract the heart from a porcine model for cardiac transplantation.
13:10
Direct Re-implantation of Left Coronary Artery into the Aorta in Adults with Anomalous Origin of Left Coronary Artery from the Pulmonary Artery (ALCAPA)
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Cited by 7 •
2017
Surgical correction of ALCAPA is highly recommended, regardless of age or the degree of intercoronary collateralization. This protocol presents a technique for the direct re-implantation of adult-type ALCAPA into the aorta to re-establish the dual-coronary perfusion. Whenever feasible, direct re-implantation is preferred to other surgical correction techniques.
08:08
Cardiopulmonary Bypass in a Mouse Model: A Novel Approach
0 Views •
Cited by 9 •
2017
This paper describes how to perform cardiopulmonary bypass in mice. This novel model will facilitate the investigation of the molecular mechanisms involved in organ damage.
FAQs
Potassium depolarizes cardiac cell membranes and produces diastolic arrest, stopping the heart in a relaxed phase. Cooling then reduces myocardial metabolic activity and oxygen demand during the period of intentionally interrupted coronary flow. Together, these effects limit the heart’s energy requirements while the surgeon works on a motionless myocardium.
Antegrade delivery and retrograde delivery provide two routes through the coronary circulation, but the central concern is whether the cold solution reaches the myocardium adequately. Distribution matters because protection depends on delivering cooling and the arrest-producing solution throughout the heart during interrupted coronary flow. If coverage is inadequate, the intended protective effect may not be uniform.
Diastolic arrest creates a still heart rather than merely lowering its temperature. That immobility gives the surgeon a motionless operative target while coronary blood flow is intentionally interrupted, and it works with reduced myocardial metabolic activity and oxygen demand to limit ischemic injury. The result is both technical access and myocardial protection during cardiac surgery.
The technique requires controlled cooling, administration of a cold cardioplegic solution through either an antegrade or retrograde coronary route, and maintenance of arrest while coronary flow is interrupted. The team must also ensure adequate distribution, control temperature, and provide timely redosing when needed. These elements connect the protective mechanism to the operative period.
Within cardiac surgery, it is used during procedures such as valve repair and coronary revascularization. In these settings, induced arrest provides the still field needed for operative work, while cooling and the solution’s composition support the myocardium during interrupted coronary flow. Its value therefore combines surgical exposure with protection against ischemic injury.
Effectiveness depends on three linked conditions: adequate distribution of the solution, appropriate temperature control, and redosing at the right time when protection needs to continue. A failure in any of these areas can weaken the intended myocardial protection during coronary interruption. These are therefore central procedural considerations, not optional refinements, when the technique is used.