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Immunology and Infection
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Establishment of Deep Hypothermic Circulatory Arrest in Rats
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Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of Deep Hypothermic Circulatory Arrest in Rats

Establishment of Deep Hypothermic Circulatory Arrest in Rats

DOI:
10.3791/63571-v

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08:39 min

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December 16, 2022

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1Department of Cardiopulmonary Bypass, National Center for Cardiovascular Disease and Fuwai Hospital, State Key Laboratory of Cardiovascular Medicine, Chinese Academy of Medical Science,Peking Union Medical College

Chapters

  • 00:05Introduction
  • 00:35Preparatory Work
  • 01:04Anesthesia and Cannulation
  • 02:54DHCA Initiation
  • 03:28Cooling
  • 03:55Deep Hypothermic Circulatory Arrest
  • 04:34Warm-Up and Reperfusion
  • 05:27Weaning off CPB
  • 05:59Results
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents the establishment of deep hypothermic circulatory arrest in rats, which can be applied to investigate systemic inflammatory response syndrome, ischemia/reperfusion injury, oxidative stress, neuroinflammation, etc.

Tags

Keywords: Deep Hypothermic Circulatory ArrestDHCARatCardiopulmonary BypassCPBAnesthesiaCannulationFemoral ArteryTail ArteryJugular VeinHeparinPriming SolutionBlood Pressure Monitoring

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