Metabolic Cart Measurements

Metabolic cart measurements are a form of indirect calorimetry that estimates energy expenditure by analyzing oxygen consumption and carbon dioxide production. A metabolic cart collects a person’s inspired and expired gases, then calculates oxygen uptake, carbon dioxide output, respiratory exchange ratio, and resting energy expenditure from gas concentrations and airflow under standardized conditions. In medicine, these measurements support individualized nutrition planning, assessment of metabolic status, and evaluation of exercise capacity or cardiopulmonary function. By providing physiologic data rather than relying only on predictive equations, metabolic cart testing can improve clinical decision-making for patients with critical illness, obesity, or other conditions affecting energy metabolism.

Metabolic Cart Measurements - Related Videos

Research

JoVE Journal - Biology

Measurement of Metabolic Rate in Drosophila using Respirometry

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Cited by 49 •

2014

Metabolic disorders are among one of the most common diseases in humans. The genetically tractable model organism D. melanogaster can be used to identify novel genes that regulate metabolism. This paper describes a relatively simple method which allows studying the metabolic rate in flies by measuring their CO2 production.

Research

JoVE Journal - Medicine
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Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants

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Cited by 49 •

2013

We combined frequency-domain near-infrared spectroscopy measures of cerebral hemoglobin oxygenation with diffuse correlation spectroscopy measures of cerebral blood flow index to estimate an index of oxygen metabolism. We tested the utility of this measure as a bedside screening tool to evaluate the health and development of the newborn brain.

Extracellular Flux Assay: A Method for Measuring Metabolic Profile of Cells

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2023

This protocol provides a biochemical approach for measuring extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of primary leukemic cells with extracellular flux assay. The assessment of the metabolic profile of these cells helps to characterize their demands, leading to personalized therapy.

Extracellular Purine Metabolism: An HPLC-based Assay to Measure Adenosine Generation and Metabolism in Leukemic Cells

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2023

This video explains a quantitative reverse phase high-performance liquid chromatography (RP-HPLC) method to measure metabolism of adenosine and associated purines generated by consumption of AMP in extracellular environment of leukemic cancer cells. The protocol describes a sensitive, specific, reliable and reproducible RP-HPLC assay developed and validated for the quantification of extracellular purine nucleotides and nucleosides produced by purified chronic lymphocytic leukemia (CLL) cells...

Research

JoVE Journal - Biology
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Metabolic Profile Analysis of Zebrafish Embryos

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Cited by 42 •

2013

Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.

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