Monofilament Mcao Procedure
Monofilament Mcao Procedure - Related Videos
09:40
Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining
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Cited by 97 •
2012
The intraluminal middle cerebral occlusion model in mice is herein presented. The extent of cerebral infarct is evaluated by a neurologic score and cresyl violet staining, an alternative staining to TTC, offering the great advantage to test in parallel many interest markers.
13:01
Intraluminal Middle Cerebral Artery Occlusion (MCAO) Model for Ischemic Stroke with Laser Doppler Flowmetry Guidance in Mice
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Cited by 76 •
2011
The intraluminal middle cerebral artery occlusion (MCAO) model is the most frequent used model among experimental ischemic stroke models. Here we will demonstrate the entire model in detail with the guide of Laser Doppler flowmetry, and its representative results.
06:38
The Stroke Preclinical Assessment Network Multi-Laboratory Model of Thromboembolic Stroke with Thrombolysis: TE-MCAo
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2025
To simulate thromboembolic stroke, emboli prepared from heterologous rat blood were injected into the middle cerebral artery, followed by administration of systemic thrombolysis for recanalization. This version of the model is optimized for use in a multi-laboratory network and supports testing multiple candidate therapeutics.
06:31
Assessment of Midline Lingual Point-Pressure Somatosensation Using Von Frey Hair Monofilaments
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Cited by 9 •
2020
This work describes a standard method to assess tactile sensation at the midline of the tongue tip. Using Von Frey Hair (VFH) monofilaments, this protocol provides estimates of detection and discrimination threshold estimates for oral point pressure (OPP).
09:31
Evaluating the Procedure for Performing Awake Cystometry in a Mouse Model
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Cited by 10 •
2017
This study describes the surgical procedures and experimental techniques for performing awake cystometry in a freely moving mouse. In addition, it provides experimental evidence to support its optimization and standardization.
FAQs
Withdrawing the nylon filament permits blood flow to return after the artery has been blocked, creating a transient ischemia model. Keeping the vessel occluded produces a different experimental condition. This distinction allows investigators to examine injury from interrupted circulation alongside responses associated with reperfusion and subsequent recovery.
Species, filament size, occlusion duration, and surgical technique can all alter the resulting ischemic injury. These variables influence how consistently the middle cerebral artery is blocked and how severe the brain response becomes. Controlling them is therefore essential when comparing neurological deficits, infarct development, or treatment effects across experiments.
The procedure focuses on the origin of the middle cerebral artery, allowing investigators to create a localized interruption of cerebral blood flow rather than a general loss of circulation. This focal arrangement supports analysis of regionally concentrated brain injury and makes it possible to relate vascular interruption to neurological impairment and infarct formation.
A transient monofilament MCAO experiment includes a later withdrawal step that permits reperfusion, whereas a permanent model does not restore flow during the study period. The two designs can therefore produce different patterns of injury and recovery. Selecting between them depends on whether the investigation emphasizes sustained ischemia or the consequences of restored circulation.
Investigators typically establish the arterial blockage, maintain it for the selected occlusion period, and, when using a transient design, withdraw the filament to permit reperfusion. They then assess neurological deficits and infarct development, while examining cellular responses to interrupted blood flow. These observations connect the surgical intervention with measurable brain outcomes.
The model can provide evidence about behavioral or neurological impairment, the development of brain infarcts, and cellular reactions to interrupted circulation. Together, these outcomes help characterize the extent of experimental brain injury and subsequent recovery. They also give researchers endpoints for comparing disease mechanisms, experimental conditions, and candidate stroke interventions.
Because the procedure produces controlled focal ischemia, researchers can evaluate whether an intervention changes neurological deficits, infarct development, or cellular responses after blood-flow interruption. The approach is used in preclinical studies of stroke therapies, but interpretation requires attention to species, filament size, occlusion duration, and surgical technique.
Results may not be directly comparable when studies use different rodent species, filament sizes, occlusion durations, or surgical approaches. Each factor can affect the severity and reproducibility of the induced injury. Careful comparison of these experimental conditions helps distinguish genuine treatment or biological effects from differences created by the model itself.