Palm Microbeam

Palm Microbeam is a specialized medical microbeam approach that concentrates therapeutic energy within microscopic, precisely defined regions of tissue. It works by directing narrowly focused beams, often as an organized pattern, so treatment intensity varies across the target and intervening tissue can retain greater functional integrity than with a uniformly distributed dose. This spatial precision supports research and potential clinical applications in targeted tissue ablation, radiation treatment, and studies of how healthy and diseased tissues respond to localized energy exposure. By linking beam geometry with biological response, Palm Microbeam methods may contribute to safer, more selective medical interventions.

Palm Microbeam - Related Videos

Research

JoVE Journal - Bioengineering

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

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Cited by 14 •

2015

Protein-protein interactions are visualized in cells with nanometer spatial resolution by combining bimolecular fluorescence complementation (BiFC) with photoactivated localization microscopy (PALM). Described here is the use of BiFC-PALM for imaging Ras-Raf interactions in U2OS cells for visualizing the nanoscale clustering and diffusion of individual Ras-Raf complexes.

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

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Cited by 1 •

2023

This protocol aimed to describe detailed guidance on the preparation of hard seed sample sections with low water content for MALDI-IMS analysis, maintaining analytes' original distribution and abundance and providing high-quality signal and spatial resolution.

Super-resolution Imaging of Neuronal Dense-core Vesicles

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Cited by 3 •

2014

We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.

Research

JoVE Journal - Biology
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Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

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Cited by 14 •

2012

We describe a method to localize fluorescently tagged proteins in electron micrographs. Fluorescence is first localized using photo-activated localization microscopy on ultrathin sections. These images are then aligned to electron micrographs of the same section.

Education

JoVE Science Education - Clinical Skills

Elbow Exam

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2023

Source: Robert E. Sallis, MD. Kaiser Permanente, Fontana, California, USA The elbow is a hinged joint that involves the articulation of 3 bones: the humerus, radius, and ulna. It is a much more stable joint than the shoulder, and because of that, the elbow has less range of motion. The elbow and its structures are prone to significant injuries, particularly with repetitive motion. Lateral and medial epicondylitis (also called tennis elbow and golfer's elbow) are two common diagnoses and often...

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