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JoVE Journal
Neuroscience

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Super-resolution Imaging of Neuronal Dense-core Vesicles
 

Super-resolution Imaging of Neuronal Dense-core Vesicles

Article DOI: 10.3791/51394
July 2nd, 2014

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Summary July 2nd, 2014

We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.

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