Porcine Thymus Retrieval

Porcine thymus retrieval is a surgical tissue-collection procedure used to obtain the thymus from a pig for medical and biomedical research. The process involves identifying thymic tissue along the cervical and thoracic regions, then carefully dissecting and removing it from surrounding structures while preserving tissue integrity and limiting contamination. Retrieved thymus samples support investigations of T-cell development, immune regulation, thymic biology, and transplantation-related questions. Standardized retrieval can improve tissue quality and experimental reproducibility, making it valuable for studies that use porcine anatomy and immune function as models of human disease and therapeutic development.

Porcine Thymus Retrieval - Related Videos

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JoVE EoE - Large Animal Models

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2025

This video demonstrates nephrectomy, the surgical procedure to remove one of the kidneys along with the section of the ureter. The blood vessels to and from the kidney are tied and divided. The excised kidney can be used for transplantation procedures.

Research

JoVE Journal - Biology
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Reaggregate Thymus Cultures

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Cited by 22 •

2008

In this video the preparation of 2-dGuo-treated reaggregate thymus cultures is demonstrated.

In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

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Cited by 21 •

2008

We present step-by-step instructions for the generation of neonatal chimeras as well as the dissection and preparation of the thymus for ex vivo imaging by 2-Photon Microscopy.

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

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Cited by 13 •

2012

We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.

Research

JoVE Journal - Biology
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Preparation of 2-dGuo-Treated Thymus Organ Cultures

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Cited by 9 •

2008

This video demonstrates the dissection and removal of the fetal thymus as well the preparation of ex vivo cultures of 2-dGuo-treated thymus.

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