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In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
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In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy

Article DOI: 10.3791/652 • 15:00 min • January 25th, 2008
January 25th, 2008 •
1, 1, 1
1Department of Molecular and Cell Biology, University of California, Berkeley

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We present step-by-step instructions for the generation of neonatal chimeras as well as the dissection and preparation of the thymus for ex vivo imaging by 2-Photon Microscopy.

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In Situ Imaging Mouse Thymus 2-photon Microscopy Thymocyte Development Migration Of Thymocytes Neonatal Chimeras Hematopoietic Chimeras Bone Marrow Injection Thymus Dissection Ex Vivo Imaging DMEM Without Phenol Red Diverse T Cell Repertoire
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