Recirculating System
Recirculating System - Related Videos
06:16
Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
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2018
We describe the assembly, operation, and cleaning of a flow apparatus designed to image fungal biofilm formation in real time while under flow. We also provide and discuss quantitative algorithms to be used on the acquired images.
09:50
Electrochemically and Bioelectrochemically Induced Ammonium Recovery
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Cited by 31 •
2015
We demonstrate the extraction of ammonium from an ammonium-rich stream using an electrochemical and a bioelectrochemical system. The reactor setup, operation and data analysis are discussed.
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
09:26
Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
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Cited by 8 •
2015
This article describes the detailed protocol and equipment necessary for dual optical mapping of transmembrane potential (Vm) and free intra-sarcoplasmic reticulum (SR) Ca2+ in the Langendorff-perfused rabbit heart. This method allows for direct observation and quantification of Vm and SR Ca2+ dynamics in the intact heart.
02:47
In Vitro Parallel-Plate Flow Model to Study Bacterial Adhesion on a Tissue Biopsy
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2026
Source: Ditkowski, B., et al. An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues. J. Vis. Exp. (2019).This video demonstrates the method of using a parallel-plate flow chamber to perfuse fluorescently labeled bacteria over vascular graft tissues, enabling controlled analysis of bacterial adhesion and early pathogenesis under shear flow conditions.
FAQs
Pump-driven circulation keeps the fluid moving through each connected module, while the treatment module performs the intended processing before the fluid returns for another pass. Tubing provides the flow path, and a reservoir accommodates the circulating fluid. This arrangement lets operators maintain a controlled pathway for repeated treatment or measurement rather than losing the fluid after one cycle.
Flow rate, pressure, temperature, and composition are the principal conditions to monitor because changes in them can alter how the circulating fluid passes through the system and how consistently it is processed. Sensors provide measurements, and adjustments to operating conditions help maintain stable processing. In medical settings, this control supports more reproducible circulation, treatment, and measurement.
A reservoir serves as a holding point within the loop while fluid continues to circulate through tubing and connected components. Its inclusion, together with sensors and a treatment module, allows the system to accommodate monitoring and processing without interrupting the overall pathway. This component arrangement is useful when blood, perfusate, medication solutions, or other fluids require controlled handling.
They are used when a medical or research process requires fluid to pass repeatedly through a controlled pathway. Examples in the source include extracorporeal circulation, organ perfusion, dialysis, drug delivery, and laboratory models of physiological transport. The appropriate use depends on whether the goal is to circulate blood, perfusate, medication solutions, or another fluid under monitored conditions.
Beyond moving fluid, the system can support repeated treatment or measurement while maintaining stable operating conditions. Monitoring flow rate, pressure, temperature, and composition provides information about how the process is behaving over time. These capabilities make the approach useful for controlled medical procedures and laboratory work in which consistency and reduced fluid loss are important.
In research, these systems can model physiological transport and support organ perfusion under controlled, monitored conditions. A circulating fluid can be processed and measured repeatedly, allowing investigators to examine system behavior without continually replacing the fluid. This makes the approach relevant to laboratory studies that investigate transport, treatment conditions, or perfusion-related processes in a controlled setting.