JoVE Encyclopedia of Experiments
Microbiology
0 views • 2:47 min • March 31st, 2026
Begin with a perforated microscope slide and place a circular tissue biopsy over the perforated area.
Next, place a rubber gasket over it.
Flip and insert this assembly into the bottom section of the metal perfusion chamber frame.
Align the top frame with the bottom and secure them.
Use a caliper to confirm the uniform chamber height.
The chamber is connected to a pump, which in turn is attached to a reservoir containing bacteria suspended in buffer.
Start the perfusion of the bacterial suspension into the chamber.
The central perforation in the slide allows the entry of the bacterial suspension into the assembly.
This enables the interaction between bacteria and the tissue surface, promoting adhesion and colonization.
This setup generates an in vitro model that mimics in vivo bacterial colonization on the tissue.
To begin the protocol, place a previously prepared tissue biopsy 10 millimeters in diameter, and the same thickness between a microscope slide and an eight millimeter circular perforation, and a rubber gasket with the inner surface facing up, to contact the bacterial suspension.
Focus on the tissue graft orientation while placed in the holder. Make sure that the inner surface of the
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