Rodent Cerebral Vessel
Rodent Cerebral Vessel - Related Videos
08:22
Permanent Cerebral Vessel Occlusion via Double Ligature and Transection
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Cited by 16 •
2013
We describe a highly reproducible method for the permanent occlusion of a rodent major cerebral blood vessel. This technique can be accomplished with very little peripheral damage, minimal blood loss, a high rate of long-term survival, and consistent infarct volume commensurate with the human clinical population.
13:16
Intravascular Perfusion of Carbon Black Ink Allows Reliable Visualization of Cerebral Vessels
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Cited by 16 •
2013
Analysis of rodent cerebrovascular anatomy plays an important role in experimental stroke research. In this context, intravascular perfusion with colored latex has been considered as a standard tool for several years. However, this technique implies distinct technical limitations, which undermine its reproducibility. Here, we describe a simple method to visualize cerebral vessels in a reproducible manner. Injection of a mixture of two commercially available carbon black inks through the left...
01:20
Congo Red Staining to Visualize Amyloid Fibrils in Cerebral Vessel Walls
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2025
The tissue shows amyloid fibrils and abnormal protein aggregates in the cerebral vessel walls, which are key indicators of neurological disorders.
02:46
In Vivo Imaging of Cerebral Vessels and Amyloid Plaques in an Alzheimer’s Disease Mouse Model
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2025
Source: Arango-Lievano, M. et. al., Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases. J. Vis. Exp. (2016)This video demonstrates in vivo two-photon imaging to visualize fluorescently labeled amyloid plaques and cerebral vasculature. Blue fluorescence marks plaques, while green fluorescence highlights the cerebral blood vessels. A 3D map of the region of interest and high-resolution mosaic imaging reveal detailed interactions of amyloid plaques with cerebral...
09:47
Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants
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Cited by 20 •
2013
The ventricular cerebrospinal fluid (CSF) bathes the neuroepithelial and cerebral cortical progenitor cells during early brain development in the embryo. Here we describe the method developed to isolate ventricular CSF from rodent embryos of different ages in order to investigate its biological function. In addition, we demonstrate our cerebral cortical explant dissection and culture technique that allows for explant growth with minimal volumes of culture medium or CSF.
FAQs
The endothelial lining, smooth muscle, and surrounding perivascular cells contribute different regulatory functions. Together, they respond to neural activity and physiological signals by adjusting vessel diameter and controlling local blood flow. Examining these coordinated responses helps researchers investigate how vascular regulation supports active brain regions and how disruptions may contribute to cerebrovascular dysfunction.
Barrier permeability indicates how effectively the vessel limits exchange between the bloodstream and brain tissue. Changes can reveal vascular dysfunction associated with inflammation, stroke, or other neurological disease mechanisms. Measuring this property also helps evaluate whether a therapeutic strategy or drug delivery approach may alter access to the brain or affect the barrier’s protective role.
Neurovascular coupling describes the relationship between neural activity and local vascular responses. When researchers examine this process, they can assess whether vessel behavior appropriately reflects changing demands from active neural tissue. Rodent cerebral vessels therefore provide a model for connecting vascular regulation with neural function and for investigating how that relationship may become impaired in disease.
Changes in vessel diameter, blood flow regulation, and blood-brain barrier permeability provide complementary indicators of dysfunction. Diameter and flow reflect vascular control, whereas permeability reflects barrier integrity. Considering these outcomes together can distinguish altered regulation from impaired protection and can clarify how inflammation, stroke, or other disease-related processes affect the neurovascular system.
Researchers use these vessels to model neurovascular coupling, inflammation, stroke, and blood-brain barrier dysfunction. The models connect cellular vascular behavior with clinically relevant neurological processes, allowing investigators to examine mechanisms that may influence disease. Their use supports medical research by providing an experimental system for studying cerebrovascular pathology before considering implications for human neurological conditions.
These models can help evaluate cerebrovascular therapies and examine strategies intended to deliver drugs to the brain. Investigators can relate treatment effects to vessel diameter, blood flow regulation, or barrier permeability. That information is useful for determining how an intervention interacts with vascular control and whether altered barrier behavior could influence access to neural tissue.