Shuttle Run
Shuttle Run - Related Videos
09:00
Cargo Loading onto Kinesin Powered Molecular Shuttles
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Cited by 4 •
2010
Molecular shuttles consisting of functionalized microtubules gliding on surface-adhered kinesin motor proteins can serve as a nanoscale transport system. Here, the assembly of a typical shuttle system is described.
08:04
Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
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Cited by 2 •
2019
We present a protocol to build molecular shuttles, where surface-adhered kinesin motor proteins propel dye-labelled microtubules. Weak interactions of the kinesins with the surface enables their reversible attachment to it. This creates a nanoscale system which exhibits dynamic assembly and disassembly of its components while retaining its functionality.
08:43
Combined Shuttle-Box Training with Electrophysiological Cortex Recording and Stimulation as a Tool to Study Perception and Learning
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Cited by 20 •
2015
Shuttle-box avoidance learning is well-established in behavioral neuroscience. This protocol describes how shuttle-box learning in rodents can be combined with site-specific electrical intracortical microstimulation (ICMS) and simultaneous chronical in vivo recordings as a tool to study multiple aspects of learning and perception.
04:25
CRISPR-based Shuttle Cloning: A High-throughput Cloning Method
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2025
We describe a protocol for a high-throughput cloning method, CRISPR-based shuttle cloning (CRISPRshuttle cloning), which allows the transfer of DNA fragments of interest between vectors without the need for PCR amplification of the DNA fragments.
03:31
Intramuscular Injections Along the Motor End Plates to Shuttle Tracers Directly into Rat Motor Neurons
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2025
Source: Mohan, R., et al. Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons. J. Vis. Exp. (2015)This video demonstrates the targeted delivery of a fluorescent neuronal tracer into motor neurons via intramuscular injection at the motor end plates (MEPs). It outlines the steps involved in preparing the rat, injecting the tracer, and assessing labeled motor neurons for neurotherapeutic potential.
FAQs
Repeated acceleration, braking, and redirection make the shuttle run more demanding than steady forward running. Each cycle requires the participant to produce speed, stop at a marked point, turn, and accelerate again while continuing for the prescribed timed effort. The resulting performance therefore reflects combined cardiovascular endurance, speed, agility, and tolerance of intermittent exertion.
Stopping and turning are not merely procedural details; they add repeated changes in movement demand. A person’s result can therefore capture more than the ability to sustain a continuous run, because the task combines locomotor control with repeated exertion. In clinical fitness assessment, this combined profile can help characterize changes in mobility and cardiorespiratory function over time.
Shuttle-run performance is best interpreted as a composite outcome rather than a single measure of speed. Differences in performance may reflect the interaction of endurance, acceleration, agility, and tolerance of intermittent effort. This matters in medicine because a change in the result may indicate a broader change in functional exercise capacity, not just one isolated physical skill.
An assessment requires a clearly marked course, a defined distance between points, and a timing arrangement for the back-and-forth runs. Participants repeatedly travel between the markers, stop, change direction, and continue for the specified timed protocol. Keeping these conditions defined supports comparison between separate assessments when monitoring physical fitness or rehabilitation progress.
In rehabilitation, clinicians can repeat the test to monitor whether functional exercise capacity changes during recovery. The result may contribute to evaluation of mobility or cardiorespiratory function and help characterize progress over time. Because the test is field based, it offers a practical option when laboratory exercise testing is impractical while providing a structured performance measure.
Shuttle Run results can inform activity recommendations by showing how a person performs during repeated, intermittent exertion. They may also help characterize recovery, fitness, or mobility changes rather than serving as a standalone description of health. In medical settings, repeating the same assessment can help reveal whether performance is changing during rehabilitation and guide follow-up decisions.