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JoVE Journal
Engineering

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Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
 

Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins

Article DOI: 10.3791/59068-v
January 26th, 2019

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Summary January 26th, 2019

We present a protocol to build molecular shuttles, where surface-adhered kinesin motor proteins propel dye-labelled microtubules. Weak interactions of the kinesins with the surface enables their reversible attachment to it. This creates a nanoscale system which exhibits dynamic assembly and disassembly of its components while retaining its functionality.

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