Single-stage Reconstruction

Single-stage reconstruction is a surgical approach that restores the form and function of damaged or removed anatomy during one planned operative episode, rather than across multiple procedures. It may use the patient’s own tissue, a prosthetic device, or a combination, selected to match the defect and provide stable coverage and support; the surgeon must also assess tissue quality, blood supply, and healing capacity before closure. In medicine, this strategy can shorten the overall treatment pathway and reduce the burden of repeated operations, while offering timely restoration after trauma, cancer treatment, or congenital abnormalities. Its success depends on appropriate patient selection, precise planning, and monitoring for complications during recovery.

Single-stage Reconstruction - Related Videos

Research

JoVE Journal - Bioengineering

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

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Cited by 12 •

2016

Tissue engineering often includes in vitro expansion in order to create autografts for tissue regeneration. In this study a method for tissue expansion, regeneration, and reconstruction in vivo was developed in order to minimize the processing of cells and biological materials outside the body.

Modified Single-Loop Reconstruction for Pancreaticoduodenectomy

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Cited by 2 •

2019

The technique of modified single-loop reconstruction following pancreaticoduodenectomy separates pancreatic secretion from bile, thus reducing the severity of POPF without prolonging the duration of surgery.

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

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Cited by 35 •

2011

This article describes a standard method to get a three-dimensional (3D) reconstruction of biological macromolecules using negative staining electron microscopy (EM). In this protocol, we explain how to get the 3D structure of the Saccharomyces cerevisiae exosome complex at medium resolution using the random conical tilt reconstruction method (RCT).

Single-stage Dynamic Reanimation of the Smile in Irreversible Facial Paralysis by Free Functional Muscle Transfer

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Cited by 17 •

2015

The use of the masseteric nerve as donor nerve represents a single-stage alternative to the criterion standard two-stage procedure of cross-facial nerve grafting and free muscle transfer in facial paralysis. We provide a detailed description to safely perform this technique with a gracilis muscle transfer and discuss indications and limitations.

Education

JoVE Core - Cell Biology

Electron Microscope Tomography and Single-particle Reconstruction

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2023

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo. Electron Tomography Electron tomography can be performed either in TEM or STEM (scanning transmission...

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