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March 28, 2011
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This article describes a standard method to get a three-dimensional (3D) reconstruction of biological macromolecules using negative staining electron microscopy (EM). In this protocol, we explain how to get the 3D structure of the Saccharomyces cerevisiae exosome complex at medium resolution using the random conical tilt reconstruction method (RCT).
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Cite this Article
Liu, X., Wang, H. Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method. J. Vis. Exp. (49), e2574, doi:10.3791/2574 (2011).
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