Surgical Injury Model
Surgical Injury Model - Related Videos
07:44
Surgical Injury to the Mouse Pancreas through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation
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Cited by 19 •
2015
This protocol describes an injury model involving the surgical ligation of the pancreatic duct in the adult mouse pancreas, resulting in severe injury that establishes an environment that allows beta cell neogenesis and proliferation. This model can be used as a tool to study mechanisms involved in beta cell formation.
11:04
Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice
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Cited by 125 •
2011
Lateral fluid percussion (LFP), an established model of traumatic brain injury in mice, is demonstrated. LFP fulfills three major criteria for animal models: validity, reliability and clinical relevance. The procedure, consisting of surgical craniotomy, fixation of hub followed by induction of injury, resulting in focal and diffuse injuries, is described.
09:09
Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice
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Cited by 141 •
2013
We describe models of moderate and severe ischemia-reperfusion-induced kidney injury in which the mice undergo unilateral renal pedicle clamping followed by simultaneous or delayed contralateral nephrectomy, respectively. These models consistently give rise to renal dysfunction and post-injury fibrosis, but injury severity and survival are dependent on mouse background, age and surgical equipment.
04:08
Mouse Model of Surgical Uterine Injury and Subsequent Pregnancy Outcomes
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2025
This protocol describes a procedure for inducing uterine injury in a mouse model, followed by downstream analyses during a subsequent pregnancy.
04:19
A Mouse Model of Single and Repetitive Mild Traumatic Brain Injury
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Cited by 16 •
2017
Athletes absorb several hundred mild traumatic brain injuries (mTBI)/concussions every year; however, the consequence of these on the brain is poorly understood. Therefore, an animal model of single and repetitive mTBI that consistently replicates clinically relevant symptoms provides the means to advance the study of mTBI and concussion.
FAQs
Researchers standardize the injury location, extent, timing, and perioperative conditions. Keeping these features consistent reduces avoidable variation between experimental groups and makes molecular, cellular, and functional findings easier to compare. This design is especially important when investigators assess whether a surgical technique, biomaterial, or therapeutic intervention changes the postoperative response rather than merely reflecting differences in the initial injury.
Evaluation combines molecular, cellular, and functional measurements rather than relying on a single outcome. This range can reveal changes in wound healing, inflammation, edema, pain, infection, or organ dysfunction at different levels of biological organization. Using several outcome types helps connect underlying tissue responses with broader physiological consequences relevant to medicine.
A controlled injury allows investigators to examine how tissues respond after an operation, including inflammatory activity and repair. Depending on the model and measurements selected, researchers can also study processes associated with wound healing, edema, pain, infection, and organ dysfunction. These observations help link postoperative changes to specific experimental conditions and potential interventions.
The core workflow begins with a defined surgical intervention in a living organism or tissue preparation. Investigators then maintain specified injury and perioperative conditions, establish the timing of observations, and collect molecular, cellular, and functional measurements. Comparing these results across standardized conditions provides a controlled basis for interpreting postoperative biology and treatment effects.
These models support testing of surgical techniques, biomaterials, and therapeutic interventions under controlled conditions. Researchers can compare postoperative responses after changing one of these factors while keeping the injury design and perioperative conditions consistent. The resulting molecular, cellular, and functional data can show whether an intervention influences repair, inflammation, edema, pain, infection, or organ dysfunction.
Ethical oversight governs the use of living organisms and helps ensure that experimental procedures are responsibly designed. Reproducible protocols, including consistent injury characteristics, timing, and perioperative conditions, strengthen confidence that observed findings reflect the factor being studied. Together, these safeguards improve the reliability of results and their relevance to patient care.